PMID- 11594382
OWN - NLM
STAT- MEDLINE
DCOM- 20020205
LR  - 20220408
IS  - 0042-4900 (Print)
IS  - 0042-4900 (Linking)
VI  - 149
IP  - 12
DP  - 2001 Sep 22
TI  - Clinical and pathological observations on pigs with postweaning multisystemic 
      wasting syndrome.
PG  - 357-61
AB  - The aim of this work was to characterise the lesions and agents present in 
      clinically normal and clinically affected pigs on a farm during an outbreak of 
      postweaning multisystemic wasting syndrome (PMWS), and to evaluate the diagnostic 
      techniques for detecting porcine circovirus type 2 (PCV-2) and other 
      microorganisms. Four pigs in the early stage and 11 pigs in the late stage of the 
      disease, and eight clinically normal pigs were necropsied. Samples of lymphoid 
      tissue and serum were also obtained from 12 slaughter pigs from the same farm. 
      The tissues were examined histopathologically, and in situ hybridisation, 
      serology and PCR were used to detect porcine circovirus type 1 (PCV-1) and/or 
      PCV-2 in tissues and/or sera. The presence of porcine reproductive and 
      respiratory syndrome virus (PRRSV), Aujeszky's disease virus (ADV) and porcine 
      parvovirus (PPV) were also investigated. Characteristic microscopical lesions of 
      PMWS were observed in the lymphoid tissues of the pigs in all three necropsied 
      groups; the lesions were most common and severe in the pigs in the early stage of 
      the disease, less so in the pigs in the late stage of the disease, and least in 
      the clinically normal pigs. PCV-2 infection was detected in all the necropsied 
      pigs by in situ hybridisation and PCR. Only three pigs had the PCV-1 genome in 
      serum or lymph node tissue. In contrast, the slaughter pigs had no microscopical 
      lesions and no PCV-2 nucleic acid in their serum or tissues, and only one of them 
      had the pCV-1 genome in its serum. Immunohistochemical, serological and PCR 
      studies revealed that PRRSV and ADV were also present on the farm during the 
      outbreak.
FAU - Quintana, J
AU  - Quintana J
AD  - Centre de Recerca en Sanitat Animal, Departament de Sanitat i Anatomia Animals, 
      Universitat Autonoma de Barcelona, Bellaterra, Spain.
FAU - Segales, J
AU  - Segales J
FAU - Rosell, C
AU  - Rosell C
FAU - Calsamiglia, M
AU  - Calsamiglia M
FAU - Rodriguez-Arrioja, G M
AU  - Rodriguez-Arrioja GM
FAU - Chianini, F
AU  - Chianini F
FAU - Folch, J M
AU  - Folch JM
FAU - Maldonado, J
AU  - Maldonado J
FAU - Canal, M
AU  - Canal M
FAU - Plana-Duran, J
AU  - Plana-Duran J
FAU - Domingo, M
AU  - Domingo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Vet Rec
JT  - The Veterinary record
JID - 0031164
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Circovirus/genetics/*pathogenicity
MH  - DNA, Viral/analysis
MH  - *Disease Outbreaks
MH  - Immunohistochemistry
MH  - In Situ Hybridization/veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Porcine respiratory and reproductive syndrome virus/isolation & 
      purification/pathogenicity
MH  - Swine
MH  - Swine Diseases/*pathology
MH  - Wasting Syndrome/pathology/*veterinary
EDAT- 2001/10/12 10:00
MHDA- 2002/02/06 10:01
CRDT- 2001/10/12 10:00
PHST- 2001/10/12 10:00 [pubmed]
PHST- 2002/02/06 10:01 [medline]
PHST- 2001/10/12 10:00 [entrez]
AID - 10.1136/vr.149.12.357 [doi]
PST - ppublish
SO  - Vet Rec. 2001 Sep 22;149(12):357-61. doi: 10.1136/vr.149.12.357.