PMID- 11595799
OWN - NLM
STAT- MEDLINE
DCOM- 20020128
LR  - 20240322
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 13
IP  - 10
DP  - 2001 Oct
TI  - Endo-beta-1,4-glucanase expression in compatible plant-nematode interactions.
PG  - 2241-55
AB  - Cyst nematodes and root-knot nematodes elaborately transform cells within the 
      vascular cylinders of plant roots into enlarged, multinucleate, and metabolically 
      active feeding cells. The giant cells of root-knot nematodes are formed by 
      repeated karyokinesis uncoupled from cytokinesis, whereas the syncytia formed by 
      cyst nematodes arise from coordinated cell wall dissolution and the coalescing of 
      cell cytoplasm of adjacent cells. Both giant cells and syncytia undergo extensive 
      cell wall architectural modifications, including thickening and the formation of 
      numerous ingrowths that increase the plasmalemma surface area for solute uptake. 
      The origin of enzymes involved in these cell wall modifications has been the 
      subject of debate for several decades. Immunolocalization of 
      endo-beta-1,4-glucanases (EGases) secreted from cyst nematodes was observed in 
      root cortical tissue during the intracellular migration of the nematodes, but 
      secretion of cyst nematode EGases into developing syncytia was not detected. We 
      have identified five EGase genes from tobacco that are upregulated within plant 
      roots upon infection by both root-knot and cyst nematodes. In situ localization 
      of tobacco EGase transcripts demonstrated that their expression was specifically 
      and developmentally upregulated within giant cells, syncytia, root tips, and 
      lateral root primordia. These data confirm that cell wall modifications within 
      plant-parasitic-nematode feeding cells arise from cell wall-modifying enzymes of 
      plant, rather than nematode, origin.
FAU - Goellner, M
AU  - Goellner M
AD  - Department of Plant Pathology, North Carolina State University, Raleigh, North 
      Carolina 27695-7616, USA.
FAU - Wang, X
AU  - Wang X
FAU - Davis, E L
AU  - Davis EL
LA  - eng
SI  - GENBANK/AF000136
SI  - GENBANK/AF077339
SI  - GENBANK/AF098292
SI  - GENBANK/AF128404
SI  - GENBANK/AF362947
SI  - GENBANK/AF362948
SI  - GENBANK/AF362949
SI  - GENBANK/AF362950
SI  - GENBANK/AF362951
SI  - GENBANK/AJ006349
SI  - GENBANK/L41046
SI  - GENBANK/M57400
SI  - GENBANK/U13054
SI  - GENBANK/U13055
SI  - GENBANK/U20590
SI  - GENBANK/U78526
SI  - GENBANK/X97189
SI  - GENBANK/X98544
SI  - GENBANK/Y11268
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - EC 3.2.1.4 (Cellulase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cellulase/*genetics
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Plant
MH  - Genes, Plant
MH  - Host-Parasite Interactions/*genetics
MH  - Molecular Sequence Data
MH  - Nematoda/*pathogenicity
MH  - Phylogeny
MH  - Plant Roots/enzymology/parasitology
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Nicotiana/classification/genetics/*parasitology
MH  - Transcription, Genetic
PMC - PMC139156
EDAT- 2001/10/12 10:00
MHDA- 2002/01/29 10:01
PMCR- 2002/10/01
CRDT- 2001/10/12 10:00
PHST- 2001/10/12 10:00 [pubmed]
PHST- 2002/01/29 10:01 [medline]
PHST- 2001/10/12 10:00 [entrez]
PHST- 2002/10/01 00:00 [pmc-release]
AID - 01-0219 [pii]
AID - 10.1105/tpc.010219 [doi]
PST - ppublish
SO  - Plant Cell. 2001 Oct;13(10):2241-55. doi: 10.1105/tpc.010219.