PMID- 11596065
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190906
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 439
IP  - 4
DP  - 2001 Oct 29
TI  - Two phases of increased cell death in the inner retina following early 
      elimination of the ganglion cell population.
PG  - 440-9
AB  - Neurons in the inner nuclear layer (INL) of the vertebrate retina undergo 
      considerable programmed cell death during development, but the determinants of 
      this cell death remain largely unknown. The present study examines the role of 
      retinal ganglion cells in support of INL neurons in the developing ferret retina. 
      The retinal ganglion cell population was eliminated by optic nerve transection at 
      postnatal day (P) 2, and the incidence of cell death was examined using terminal 
      deoxytransferase dUTP nick-end labelling (TUNEL) at various ages during the first 
      3 postnatal weeks. Significant increases in TUNEL-positive cells were observed in 
      the neuroblast layer (NBL) as early as P3, prior to synapse formation within the 
      inner plexiform layer (IPL), and again in the INL at P22, the normal peak of 
      naturally occurring cell death within the ferret's INL. A decrease in 
      TUNEL-positive cells was found in the NBL at P8. These results show three phases 
      of response to the loss of retinal ganglion cells and suggest that cells in the 
      NBL/INL are normally dependent on retinal ganglion cells for their survival. 
      Recent studies have shown that certain populations of retinal neurons are reduced 
      in adult animals that had lost the population of ganglion cells during early 
      development, so the present study also examined when this reduction could first 
      be detected. The number of parvalbumin-immunoreactive amacrine cells was 
      decreased significantly in the NBL of the manipulated eye as early as P8, when we 
      could first label this population, and this difference persisted through 
      adulthood. The fact that cell death in the NBL has already increased within 24 
      hours of ganglion cell elimination, coupled with the specificity of this effect 
      on the adult complement of INL cell types, shows that cell-cell interactions 
      controlling survival are already highly specific for particular types of retinal 
      neuron early in development
CI  - Copyright 2001 Wiley-Liss, Inc.
FAU - Cusato, K
AU  - Cusato K
AD  - Neuroscience Research Institute and Department of Psychology, University of 
      California at Santa Barbara, Santa Barbara, California 93106-5060, USA.
FAU - Stagg, S B
AU  - Stagg SB
FAU - Reese, B E
AU  - Reese BE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
SB  - IM
MH  - Animals
MH  - Animals, Newborn/growth & development/*metabolism
MH  - Apoptosis/*physiology
MH  - Cell Count
MH  - Cell Death/physiology
MH  - Ferrets
MH  - In Situ Nick-End Labeling
MH  - Optic Nerve Injuries/*metabolism
MH  - Retina/metabolism
MH  - Retinal Ganglion Cells/*metabolism
EDAT- 2001/10/12 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/12 10:00
PHST- 2001/10/12 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/12 10:00 [entrez]
AID - 10.1002/cne.1361 [pii]
AID - 10.1002/cne.1361 [doi]
PST - ppublish
SO  - J Comp Neurol. 2001 Oct 29;439(4):440-9. doi: 10.1002/cne.1361.