PMID- 11597118
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20191025
IS  - 0190-2148 (Print)
IS  - 0190-2148 (Linking)
VI  - 27
IP  - 7
DP  - 2001 Oct-Nov
TI  - Programmed cell death in normal fetal rat lung development.
PG  - 569-87
AB  - Lung development is a coordinated process regulated by the interactions of 
      extracellular and intracellular factors, yet little is known about the process of 
      programmed cell death during lung development. To study this question, we 
      examined fetal rat lung from the pseudoglandular period (gestational day 15) to 
      the day of birth (gestational day 21) using BrdU incorporation into DNA as a 
      proliferative marker, while in parallel examining several markers of programmed 
      cell death including terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL), DNA "laddering, " and expression of programmed cell death 
      pathway proteins. Cell proliferation was ongoing throughout fetal days 15 to 21 
      with a decrease in proliferation over days 20 and 21. Programmed cell death in 
      fetal lung also appeared to be present at all ages examined, but demonstrated 2 
      peaks of activity at fetal days 15 and 18 to 20. Bcl-XL expression was detected 
      on fetal days 15 to 21, with diminished expression on days E15 to E18. Cleaved 
      poly(ADP-ribose)polymerase (PARP), activated caspase-3, Bax, and Bad were 
      increased on days 18 to 20. We conclude that proliferation is the primary process 
      driving fetal lung development with programmed cell death occurring throughout 
      the lung developmental process to refine structural remodeling.
FAU - Stiles, A D
AU  - Stiles AD
AD  - Department of Pediatrics, University of North Carolina at Chapel Hill, 27599, 
      USA.
FAU - Chrysis, D
AU  - Chrysis D
FAU - Jarvis, H W
AU  - Jarvis HW
FAU - Brighton, B
AU  - Brighton B
FAU - Moats-Staats, B M
AU  - Moats-Staats BM
LA  - eng
GR  - HL19171-18 SCOR/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Bad protein, rat)
RN  - 0 (Bax protein, rat)
RN  - 0 (Bcl2l1 protein, rat)
RN  - 0 (Carrier Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (bcl-Associated Death Protein)
RN  - 0 (bcl-X Protein)
RN  - 9007-49-2 (DNA)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 3.4.22.- (Casp3 protein, rat)
RN  - EC 3.4.22.- (Caspase 3)
RN  - EC 3.4.22.- (Caspases)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Blotting, Western
MH  - Bromodeoxyuridine/metabolism
MH  - Carrier Proteins/metabolism
MH  - Caspase 3
MH  - Caspases/metabolism
MH  - Cell Division/physiology
MH  - DNA/analysis
MH  - Embryonic and Fetal Development
MH  - Gestational Age
MH  - Immunoenzyme Techniques
MH  - In Situ Nick-End Labeling
MH  - Lung/*embryology/metabolism
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Rats
MH  - bcl-2-Associated X Protein
MH  - bcl-Associated Death Protein
MH  - bcl-X Protein
EDAT- 2001/10/13 10:00
MHDA- 2002/03/01 10:01
CRDT- 2001/10/13 10:00
PHST- 2001/10/13 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2001/10/13 10:00 [entrez]
AID - 10.1080/019021401753181836 [doi]
PST - ppublish
SO  - Exp Lung Res. 2001 Oct-Nov;27(7):569-87. doi: 10.1080/019021401753181836.