PMID- 11597121
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20220330
IS  - 0190-2148 (Print)
IS  - 0190-2148 (Linking)
VI  - 27
IP  - 7
DP  - 2001 Oct-Nov
TI  - TNF-alpha enhanced allergic sensitization to house dust mite in brown Norway 
      rats.
PG  - 617-35
AB  - We have recently demonstrated that pulmonary exposure to residual oil fly ash 
      (ROFA) resulted in enhanced sensitization to house dust mite (HDM) and augmented 
      the development of allergic lung disease after allergen challenge. This effect 
      was associated with increased tumor necrosis factor alpha (TNF-alpha), a 
      macrophage- and epithelial cell-derived cytokine that promotes granulocyte 
      migration to the lung. The present study examined whether exogenous 
      administration of TNF-alpha enhances sensitization to HDM. One day prior to 
      pulmonary sensitization with 10 microg HDM (5 microg each on days 1 and 3), 
      female Brown Norway rats were instilled via the trachea with either 2.0 microg 
      recombinant rat TNF-alpha, 2.0 microg bovine serum albumin (BSA), or 1,000 microg 
      ROFA, and were challenged with 10 microg HDM 14 days later. Antigen-induced 
      immediate bronchoconstriction responses, antigen-specific immunoglobulin E (IgE) 
      titers, lymphocyte proliferation, (cytokines (TNF-alpha and interleukin [IL]-13), 
      and eosinophils were elevated in rats treated with ROFA or TNF-alpha compared 
      with BSA-treated controls after HDM challenge. Intratracheal administration of 
      anti-TNF-alpha monoclonal antibody during ROFA exposure did not reduce 
      ROFA-enhanced lymphocyte proliferation or IgE titers, but had a trend for reduced 
      pulmonary inflammation. This study demonstrates that TNF-alpha has similar 
      adjuvant activity as ROFA, but other factors may fulfill this function when 
      TNF-alpha activity is blocked.
FAU - Lambert, A L
AU  - Lambert AL
AD  - University of North Carolina at Chapel Hill, USA.
FAU - Selgrade, M K
AU  - Selgrade MK
FAU - Winsett, D W
AU  - Winsett DW
FAU - Gilmour, M I
AU  - Gilmour MI
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Coal Ash)
RN  - 0 (Cytokines)
RN  - 0 (Glycoproteins)
RN  - 0 (Particulate Matter)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7440-44-0 (Carbon)
SB  - IM
MH  - Animals
MH  - Antibodies, Blocking/pharmacology
MH  - Antibodies, Monoclonal/pharmacology
MH  - Antigens, Dermatophagoides
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Bronchoconstriction/drug effects
MH  - Carbon/adverse effects/immunology
MH  - Coal Ash
MH  - Cytokines/analysis/genetics
MH  - Disease Models, Animal
MH  - Female
MH  - Glycoproteins/*immunology
MH  - Hypersensitivity, Immediate/*immunology
MH  - Immunoglobulin E/biosynthesis
MH  - Intubation, Intratracheal
MH  - Lymphocyte Activation/drug effects
MH  - Mites/*immunology
MH  - Particulate Matter
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred BN
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2001/10/13 10:00
MHDA- 2002/03/01 10:01
CRDT- 2001/10/13 10:00
PHST- 2001/10/13 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2001/10/13 10:00 [entrez]
AID - 10.1080/019021401753181863 [doi]
PST - ppublish
SO  - Exp Lung Res. 2001 Oct-Nov;27(7):617-35. doi: 10.1080/019021401753181863.