PMID- 11597593
OWN - NLM
STAT- MEDLINE
DCOM- 20020114
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 916
IP  - 1-2
DP  - 2001 Oct 19
TI  - GDNF is a major component of trophic activity in DA-depleted striatum for 
      survival and neurite extension of DAergic neurons.
PG  - 76-84
AB  - Extracts from dopamine (DA)-depleted striatal tissue (lesion extract) and from 
      intact striatal tissue (intact extract) were prepared, and trophic activities in 
      these extracts were evaluated using survival and neurite extension of DAergic 
      neurons as indices. Levels of brain-derived neurotrophic factor (BDNF), basic 
      fibroblast growth factor (bFGF), glial cell-line derived neurotrophic factor 
      (GDNF) and neurotrophin-3 (NT-3) in extracts were measured using enzyme-linked 
      immunosorbent assay (ELISA). The lesion extract exhibited a stronger trophic 
      activity on survival and neurite extension of DAergic neurons than intact 
      extract. In lesion extract, bFGF was slightly and GDNF was significantly 
      increased, while BDNF and NT-3 were the same level in each extract. The peak 
      increase of bFGF and GDNF was during 2 to 3 weeks after DA depletion. Trophic 
      activity of extract was strongly attenuated after immunoprecipitation of GDNF and 
      partly attenuated after immunoprecipitation of bFGF. In parallel 
      immunohistological study, no significant variations were found for striatal 
      microtubule-associated protein-2 (MAP-2)- nor OX-41-immunoreactive cells, while 
      the number of strongly labeled glial fibrillary acidic protein 
      (GFAP)-immunoreactive cells were increased in DA-depleted striatum, suggesting 
      reactive gliosis. Data suggest that bFGF is a minor, while GDNF is a major 
      component of trophic activity for DAergic neurons in DA-depleted striatum, and 
      increased bFGF and GDNF levels may be mediated partly by reactive gliosis.
FAU - Nakajima, K
AU  - Nakajima K
AD  - Department of Physiology, Nagoya City University Medical School, Kawasumi 1, 
      Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan. jaface@ylw.mmtr.or.jp
FAU - Hida, H
AU  - Hida H
FAU - Shimano, Y
AU  - Shimano Y
FAU - Fujimoto, I
AU  - Fujimoto I
FAU - Hashitani, T
AU  - Hashitani T
FAU - Kumazaki, M
AU  - Kumazaki M
FAU - Sakurai, T
AU  - Sakurai T
FAU - Nishino, H
AU  - Nishino H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cell Extracts)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9005-49-6 (Heparin)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cell Differentiation/drug effects/physiology
MH  - Cell Extracts/chemistry/pharmacology
MH  - Cell Survival/drug effects/*physiology
MH  - Cells, Cultured/cytology/drug effects/metabolism
MH  - Dopamine/*deficiency
MH  - Fibroblast Growth Factor 2/metabolism/pharmacology
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Heparin
MH  - Immunohistochemistry
MH  - Microtubule-Associated Proteins/metabolism
MH  - Neostriatum/metabolism/physiopathology
MH  - *Nerve Growth Factors
MH  - Nerve Tissue Proteins/*metabolism/pharmacology
MH  - Neurites/*drug effects/metabolism/ultrastructure
MH  - Neuroprotective Agents/*metabolism/pharmacology
MH  - Neurotrophin 3/metabolism
MH  - Parkinson Disease/drug therapy/*metabolism/physiopathology
MH  - Rats
MH  - Rats, Wistar
MH  - Substantia Nigra/*drug effects/growth & development/metabolism
EDAT- 2001/10/13 10:00
MHDA- 2002/01/15 10:01
CRDT- 2001/10/13 10:00
PHST- 2001/10/13 10:00 [pubmed]
PHST- 2002/01/15 10:01 [medline]
PHST- 2001/10/13 10:00 [entrez]
AID - S0006-8993(01)02866-9 [pii]
AID - 10.1016/s0006-8993(01)02866-9 [doi]
PST - ppublish
SO  - Brain Res. 2001 Oct 19;916(1-2):76-84. doi: 10.1016/s0006-8993(01)02866-9.