PMID- 11598148
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 81
IP  - 10
DP  - 2001 Oct
TI  - Transcription of cytokeratins 8, 18, and 19 in bone marrow and limited expression 
      of cytokeratins 7 and 20 by carcinoma cells: inherent limitations for RT-PCR in 
      the detection of isolated tumor cells.
PG  - 1351-61
AB  - The suitability of "real-time" quantitative reverse transcriptase polymerase 
      chain reaction (RT-PCR) for the detection of isolated carcinoma cells in bone 
      marrow was investigated by evaluating the expression of cytokeratin (CK)7, CK8, 
      CK18, CK19, and CK20 in 17 gastrointestinal cancer cell lines, 64 control bone 
      marrow specimens from noncancer patients, and 30 bone marrow specimens from 
      patients with gastric or colorectal cancer. RT-PCR products for CK8 and CK18 were 
      detected in all cancer cell lines, but only 16, 5, and 11 cell lines provided 
      evidence for CK19, CK7, and CK20 transcription. Variable numbers of bone marrow 
      specimens from noncancer patients demonstrated background transcription of CK8 
      (78.1%), CK18 (95.3%), CK19 (35.9%), CK20 (29.6%), and CK7 (16.7%). Maximal 
      background transcription for CK8, CK18, and CK19 ranged from 52.2 to 56.1 
      copies/10(3) copies glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the 
      corresponding values of 0.06 and 0.76 copies for CK7 and CK20 being distinctly 
      lower. When maximal background values were used as a threshold value to define 
      positivity in tumor cell dilution experiments, sensitivity levels of one tumor 
      cell in 10(4) bone marrow cells were determined for CK7 and CK20 RT-PCR assays. 
      Maximal background expression values of the different CKs as obtained in the 
      control series were exceeded once (CK20), twice (CK18 and CK19), and 18 times 
      (CK7) in bone marrow specimens from cancer patients, with none of these specimens 
      exceeding the maximal background expression value of CK8. We conclude that RT-PCR 
      for CK8, CK18, and CK19 cannot be recommended for the detection of isolated tumor 
      cells in bone marrow of cancer patients. On the other side, the limited number of 
      gastric and colorectal cancer cell lines expressing CK7 and CK20 indicates that 
      assay sensitivity for these CKs might be limited because of their selective 
      expression by carcinoma cells.
FAU - Dimmler, A
AU  - Dimmler A
AD  - Institute of Pathology, University of Erlangen-Nurnberg, Erlangen, Germany.
FAU - Gerhards, R
AU  - Gerhards R
FAU - Betz, C
AU  - Betz C
FAU - Gunther, K
AU  - Gunther K
FAU - Reingruber, B
AU  - Reingruber B
FAU - Horbach, T
AU  - Horbach T
FAU - Baumann, I
AU  - Baumann I
FAU - Kirchner, T
AU  - Kirchner T
FAU - Hohenberger, W
AU  - Hohenberger W
FAU - Papadopoulos, T
AU  - Papadopoulos T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*metabolism/pathology
MH  - Gene Expression Regulation
MH  - Humans
MH  - Keratins/*biosynthesis/genetics
MH  - Neoplasms/genetics/*metabolism/pathology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2001/10/13 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/13 10:00
PHST- 2001/10/13 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/13 10:00 [entrez]
AID - S0023-6837(22)03157-9 [pii]
AID - 10.1038/labinvest.3780349 [doi]
PST - ppublish
SO  - Lab Invest. 2001 Oct;81(10):1351-61. doi: 10.1038/labinvest.3780349.