PMID- 11598832
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20081121
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 184
IP  - 9
DP  - 2001 Nov 1
TI  - Serum of cytomegalovirus-infected mice induces monocyte chemoattractant protein-1 
      expression by endothelial cells.
PG  - 1109-13
AB  - Inflammation plays a central role in atherogenesis. It was hypothesized that 
      infection of apolipoprotein E-deficient mice with murine cytomegalovirus (MCMV) 
      increases serum levels of proinflammatory cytokines, which may induce 
      "proatherosclerotic" changes in endothelial cells (ECs). Serum samples were 
      collected from uninfected and infected mice. ELISA was used to determine cytokine 
      serum levels and monocyte chemoattractant protein-1 (MCP-1) levels in the 
      supernatant of mouse ECs incubated with serum-containing medium. Serum samples 
      from infected mice induced MCP-1 expression by ECs. These serum samples contain 
      interferon (IFN)-gamma, whereas IFN-gamma was undetectable in serum samples from 
      uninfected mice. Preincubating infected mouse serum with anti-IFN-gamma 
      monoclonal antibody significantly decreased serum-induced EC expression of MCP-1. 
      Thus, MCMV infection increases IFN-gamma serum levels, such serum can induce 
      MCP-1 in ECs, and the serum-induced MCP-1 expression is due, at least in part, to 
      IFN-gamma. If these changes in EC function also occur in vivo in response to 
      infection, they could exacerbate atherogenesis.
FAU - Rott, D
AU  - Rott D
AD  - Cardiovascular Research Institute, Medstar Research Institute, Washington 
      Hospital Center, 110 Irving Street NW, Washington, DC 20010, USA.
FAU - Zhu, J
AU  - Zhu J
FAU - Burnett, M S
AU  - Burnett MS
FAU - Zhou, Y F
AU  - Zhou YF
FAU - Wasserman, A
AU  - Wasserman A
FAU - Walker, J
AU  - Walker J
FAU - Epstein, S E
AU  - Epstein SE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20010919
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antibodies, Viral)
RN  - 0 (Apolipoproteins E)
RN  - 0 (Chemokine CCL2)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Aorta
MH  - Apolipoproteins E/deficiency
MH  - Arteriosclerosis/*etiology/physiopathology
MH  - Chemokine CCL2/*biosynthesis
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Herpesviridae Infections/complications/*immunology/virology
MH  - Interferon-gamma/*blood
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Muromegalovirus/genetics/*immunology/isolation & purification
MH  - Polymerase Chain Reaction
EDAT- 2001/10/13 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/13 10:00
PHST- 2001/05/23 00:00 [received]
PHST- 2001/07/18 00:00 [revised]
PHST- 2001/10/13 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/13 10:00 [entrez]
AID - JID010582 [pii]
AID - 10.1086/323745 [doi]
PST - ppublish
SO  - J Infect Dis. 2001 Nov 1;184(9):1109-13. doi: 10.1086/323745. Epub 2001 Sep 19.