PMID- 11599126
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20161124
IS  - 0023-2513 (Print)
IS  - 0023-2513 (Linking)
VI  - 47
IP  - 2
DP  - 2001 Apr
TI  - Induction of heat shock proteins and its effects on glial differentiation in rat 
      C6 glioblastoma cells.
PG  - 77-95
AB  - Heat shock proteins (HSPs) are immediately expressed in neuronal and glial cells 
      under various stressful conditions and play a protective role through molecular 
      chaperones. We investigated the characteristics of the induction manner of heme 
      oxygenase-1 (HO-1) and HSP70 in rat C6 glioblastoma cells. In heat treatment (42 
      degrees C for 30 min), C6 cells expressed high level of HO-1 and HSP70 mRNAs 
      within 30-60 min, and their proteins at 3 hrs. Heat-induced expressions of HSPs 
      mRNAs were completely inhibited with actinomycin D, suggesting the 
      transcriptional regulation. Oxygen-glucose deprivation (OGD), cystine-free 
      (inhibition of synthesis of glutathione), cyto-toxic (ethanol, sodium butyrate) 
      treatments resulted in different expression manners between HO-1 and HSP70, which 
      suggested that HO-1 and HSP70 play different protective roles against a variety 
      kind of stressful conditions in glial cells. C6 cells can differentiate toward 
      both astrocyte and oligodendrocyte directions. Treatment with dibutyryl cyclic 
      AMP (cAMP) induces expression of glial fibrillary acidic protein (GFAP), a marker 
      of astrocytes, and treatment with retinoic acid (RA) induces expression of myelin 
      proteolipid protein (PLP), a marker of oligodendrocytes, respectively. Heat 
      treatment before the initiation of differentiation by RA reduced the RA-induced 
      expression of PLP mRNA profoundly, but not in GFAP mRNA level induced by cAMP. 
      Heat treatment after the initiation of differentiation by cAMP or RA accelerated 
      the expression of GFAP or PLP mRNAs. Astroglial differentiation by cAMP reduced 
      the heat-induced expressions of HSPs mRNAs, but no change with RA pre-treatment. 
      These results suggested that HSPs may modulate the glial differentiation in the 
      developing brain. On the contrary, glial differentiation may give influence on 
      the stress-induced HSPs expression. The timing of stressful damages, resulting in 
      the expression of HSPs, on the developing brain is critically important for the 
      pathogenesis of glial lesion. In the heat-treated C6 cells, the expression of 
      platelet-derived growth factor (PDGF) receptor-alpha mRNA was significantly 
      decreased. HSPs may have ability to induce the glial differentiation in part 
      through down-regulation of the PDGF pathway.
FAU - Zhang, W L
AU  - Zhang WL
AD  - Department of Pediatrics, Kobe University School of Medicine.
FAU - Tsuneishi, S
AU  - Tsuneishi S
FAU - Nakamura, H
AU  - Nakamura H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Kobe J Med Sci
JT  - The Kobe journal of medical sciences
JID - 0413531
RN  - 0 (Culture Media)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Myelin Proteolipid Protein)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (RNA, Messenger)
RN  - 0 (platelet-derived growth factor A)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 5688UTC01R (Tretinoin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 2.7.10.1 (Receptors, Platelet-Derived Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - *Cell Differentiation/drug effects
MH  - Culture Media
MH  - Cyclic AMP/pharmacology
MH  - Dactinomycin/pharmacology
MH  - Gene Expression/drug effects
MH  - Glial Fibrillary Acidic Protein/genetics
MH  - Glioblastoma
MH  - HSP70 Heat-Shock Proteins/genetics
MH  - Heat-Shock Proteins/*biosynthesis/*physiology
MH  - Heme Oxygenase (Decyclizing)/biosynthesis/genetics
MH  - Heme Oxygenase-1
MH  - Hot Temperature
MH  - Kinetics
MH  - Myelin Proteolipid Protein/genetics
MH  - Neuroglia/*cytology
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - Platelet-Derived Growth Factor/genetics
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Receptors, Platelet-Derived Growth Factor/genetics
MH  - Tretinoin/pharmacology
MH  - Tumor Cells, Cultured
EDAT- 2001/10/16 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/10/16 10:00
PHST- 2001/10/16 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/10/16 10:00 [entrez]
PST - ppublish
SO  - Kobe J Med Sci. 2001 Apr;47(2):77-95.