PMID- 11600319 OWN - NLM STAT- MEDLINE DCOM- 20011213 LR - 20200106 IS - 0736-5748 (Print) IS - 0736-5748 (Linking) VI - 19 IP - 6 DP - 2001 Oct TI - Brain-derived neurotrophic factor and TrkB tyrosine kinase receptor gene expression in zebrafish embryo and larva. PG - 569-87 AB - The genes that encode the neurotrophin family of secreted polypeptides and the Trk family of high affinity neurotrophin transmembrane protein tyrosine kinase receptors are induced at the time of neurogenesis in mammals and are known to play critical roles in nervous system development. We show here that in contrast to mammals, the genes encoding the neurotrophin brain-derived neurotrophic factor (BDNF) and the neurotrophin receptor TrkB are expressed throughout embryonic development in the zebrafish. At the embryonic stages preceding transcription of endogenous genes all cells contain BDNF transcripts and immunoreactive BDNF and the trkB transcripts lack the region that encodes a kinase domain. As development proceeds, progressively fewer cells contain BDNF transcripts and by the time of neurogenesis the trkB transcripts encode a kinase-domain. In the 4-day-old larva, a small subset of specialized sensory cells on the surface and cells in deeper structures including the gill arches, fin, and cloaca express the BDNF gene at high levels in a promoter-specific fashion. This progressive restriction of BDNF gene expression must involve an extinction of BDNF gene transcription in some and induction of high levels of transcription in a promoter-specific fashion in other cells. FAU - Lum, T AU - Lum T AD - Medical Service, Northern California Health Care System, 150 Muir Road, Martinez, CA 94553, USA. FAU - Huynh, G AU - Huynh G FAU - Heinrich, G AU - Heinrich G LA - eng PT - Journal Article PL - United States TA - Int J Dev Neurosci JT - International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience JID - 8401784 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Protein Isoforms) RN - 0 (RNA, Messenger) RN - EC 2.7.10.1 (Receptor, trkB) RN - EC 2.7.10.1 (Receptor, trkC) SB - IM MH - Aging/genetics MH - Animals MH - Blastocyst/cytology/metabolism MH - Blastomeres/cytology/metabolism MH - Brain-Derived Neurotrophic Factor/*genetics/metabolism MH - Cell Differentiation/*genetics MH - Cross Reactions/immunology MH - Embryo, Mammalian/cytology/embryology/metabolism MH - Embryo, Nonmammalian MH - Gene Expression Regulation, Developmental/*physiology MH - Immunohistochemistry MH - Larva/cytology/genetics/metabolism MH - Mammals/immunology MH - Nervous System/cytology/*embryology/metabolism MH - Protein Isoforms/genetics MH - Protein Structure, Tertiary/genetics MH - RNA, Messenger/*metabolism MH - Receptor, trkB/*genetics/metabolism MH - Receptor, trkC/genetics MH - Reverse Transcriptase Polymerase Chain Reaction MH - Somites/cytology/metabolism MH - Transcription, Genetic/physiology MH - Zebrafish/*embryology/genetics/metabolism EDAT- 2001/10/16 10:00 MHDA- 2002/01/05 10:01 CRDT- 2001/10/16 10:00 PHST- 2001/10/16 10:00 [pubmed] PHST- 2002/01/05 10:01 [medline] PHST- 2001/10/16 10:00 [entrez] AID - S0736574801000417 [pii] AID - 10.1016/s0736-5748(01)00041-7 [doi] PST - ppublish SO - Int J Dev Neurosci. 2001 Oct;19(6):569-87. doi: 10.1016/s0736-5748(01)00041-7.