PMID- 11601607
OWN - NLM
STAT- MEDLINE
DCOM- 20020418
LR  - 20191210
IS  - 0175-7598 (Print)
IS  - 0175-7598 (Linking)
VI  - 56
IP  - 5-6
DP  - 2001 Sep
TI  - A system of categorizing enzyme-cell wall associations in Agaricus bisporus, 
      using operational criteria.
PG  - 613-22
AB  - Enzymes were investigated for their occurrence in the cell wall fraction (4,000 g 
      sediment of the homogenate) of Agaricus bisporus sporocarps. Besides the markers 
      malate dehydrogenase (MalDH), hexokinase (HK) and ATPase, the range of entities 
      studied included gamma-glutamyl transferase (gamma-GT), mannitol dehydrogenase 
      (MDH), phenoloxidase, chitin and beta-1,3-glucan synthases (ChS, beta-GS), 
      chitinase, beta-N-acetylhexosaminidase (HexNAc'ase) and beta-glucanase. Using the 
      extractability in dilute buffer, digitonin and NaCl at high ionic strength as the 
      operational criteria, four categories (I-IV) of enzyme-wall associations could be 
      discerned: category I encompasses enzymes which are artefactually present (i.e. 
      contaminants); category II, enzymes that are hydrophobically bound (which may or 
      may not be genuinely wall-associated), III includes enzymes that are ionically 
      bound and IV, enzymes whose bonding to the wall is in all probability covalent. 
      The same enzyme entity may have representatives in more than one category, e.g. 
      ChS and beta-GS (I, II, IV), phenolase (I, II, III, IV), beta-glucanase, 
      chitinase and HexNAc'ase (I, IV). It is thought that the categorization presented 
      could be of general applicability in fungi as well as in higher plants to specify 
      enzyme-wall associations in a straightforward, comparable manner, thus avoiding 
      some of the ambiguous terms prevailing in the literature, such as "weakly", 
      "strongly" or "tightly" wall bound. The results are discussed in more detail for 
      several of the more economically important enzymes studied.
FAU - Sassoon, J
AU  - Sassoon J
AD  - Department of Chemistry and Biochemistry, University of Berne, Switzerland. 
      sassoon@ibc.unibe.ch
FAU - Mooibroek, H
AU  - Mooibroek H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Enzymes)
SB  - IM
MH  - Agaricus/*enzymology
MH  - Cell Wall/*enzymology
MH  - Enzymes/*classification/isolation & purification/*metabolism
MH  - Mycology/methods
MH  - Subcellular Fractions/enzymology
RF  - 115
EDAT- 2001/10/17 10:00
MHDA- 2002/04/19 10:01
CRDT- 2001/10/17 10:00
PHST- 2001/10/17 10:00 [pubmed]
PHST- 2002/04/19 10:01 [medline]
PHST- 2001/10/17 10:00 [entrez]
AID - 10.1007/s002530100714 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2001 Sep;56(5-6):613-22. doi: 10.1007/s002530100714.