PMID- 11601706
OWN - NLM
STAT- MEDLINE
DCOM- 20011101
LR  - 20230307
IS  - 0362-028X (Print)
IS  - 0362-028X (Linking)
VI  - 64
IP  - 10
DP  - 2001 Oct
TI  - A rapid assay for detecting sulfonamides in tissues of slaughtered animals.
PG  - 1565-73
AB  - Governments regulate antimicrobial residues in slaughtered animals with 
      surveillance programs for detecting drugs in food-producing animals. Although 
      initial screening bioassay systems are recognized for their sensitivities to 
      antimicrobial drug groups, none are sensitive to sulfonamides at or near the 
      maximum residue levels (MRLs) in the Codex Alimentarious. We have developed a 
      sulfonamide-sensitive rapid assay using Bacillus stearothermophilus inoculated PM 
      indicator agar containing bromcresol purple and trimethoprim, where the end point 
      is a combination of color change in the agar and zone of microbial growth 
      inhibition around the sampling disk. Five sulfonamides, plus 16 other 
      antimicrobial drugs were tested in standard concentrations in water, bovine 
      kidney, and ground beef. Sulfonamides were detected at concentrations near the 
      MRLs, and they were presumptively identified using para-aminobenzoic acid. The 
      rapid assay was extremely sensitive to beta-lactams that were presumptively 
      identified using penase. The system also was sensitive to tetracyclines, 
      aminoglycosides, and macrolides, of which tetracyclines and gentamicin were 
      identified using enzyme-linked immunosorbent assay (ELISA). In trials on 
      slaughterhouse tissues submitted for testing in Ontario's meat surveillance 
      program, the rapid assay identified twofold the number of positive kidneys and 
      threefold the number of positive diaphragm samples compared to a standard 
      microbiological inhibition test (MIT) currently approved. Fifty-three of 471 
      carcasses were sulfonamide positive with the rapid assay, while no sulfonamides 
      were detected with the MIT. ELISA and thin-layer chromatography were used on 
      selected samples to confirm the rapid assay sulfonamide presumptive results.
FAU - Braham, R
AU  - Braham R
AD  - Department of Biomedical Sciences, University of Guelph, Ontario, Canada.
FAU - Black, W D
AU  - Black WD
FAU - Claxton, J
AU  - Claxton J
FAU - Yee, A J
AU  - Yee AJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Food Prot
JT  - Journal of food protection
JID - 7703944
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Sulfonamides)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents
MH  - Cattle
MH  - Chromatography, Thin Layer
MH  - Drug Residues/*analysis
MH  - Food Analysis
MH  - Immunoassay/*methods
MH  - Immunoenzyme Techniques
MH  - Meat Products/*analysis
MH  - Sensitivity and Specificity
MH  - Sulfonamides/*analysis
MH  - Time Factors
MH  - Tissue Distribution
EDAT- 2001/10/17 10:00
MHDA- 2001/11/03 10:01
CRDT- 2001/10/17 10:00
PHST- 2001/10/17 10:00 [pubmed]
PHST- 2001/11/03 10:01 [medline]
PHST- 2001/10/17 10:00 [entrez]
AID - S0362-028X(22)00732-3 [pii]
AID - 10.4315/0362-028x-64.10.1565 [doi]
PST - ppublish
SO  - J Food Prot. 2001 Oct;64(10):1565-73. doi: 10.4315/0362-028x-64.10.1565.