PMID- 11602772
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20200217
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 82
IP  - Pt 11
DP  - 2001 Nov
TI  - High frequency RNA recombination in porcine reproductive and respiratory syndrome 
      virus occurs preferentially between parental sequences with high similarity.
PG  - 2615-2620
LID - 10.1099/0022-1317-82-11-2615 [doi]
AB  - Two types of porcine reproductive and respiratory syndrome virus (PRRSV) exist, a 
      North American type and a European type. The co-existence of both types in some 
      countries, such as Denmark, Slovakia and Canada, creates a risk of inter-type 
      recombination. To evaluate this risk, cell cultures were co-infected with either 
      a North American and a European type of PRRSV or two diverse types of European 
      isolate. Subsequently, an approximately 600 bp region of the PRRSV genome was 
      tested for recombination by quantitative real-time RT-PCR. Between 0.1 and 2.5% 
      RNA recombination was found between the European isolates, but no recombination 
      was detected between the European and North American types. Calculation of the 
      maximum theoretical risk of European-American recombination, based on the 
      sensitivity of the RT-PCR system, revealed that RNA recombination between the 
      European and North American types of PRRSV is at least 10 000 times less likely 
      to occur than RNA recombination between diverse European isolates.
FAU - van Vugt, Joke J F A
AU  - van Vugt JJFA
AD  - Danish Veterinary Institute for Virus Research, Lindholm, DK-4771 Kalvehave, , 
      Denmark1.
FAU - Storgaard, Torben
AU  - Storgaard T
AD  - Danish Veterinary Institute for Virus Research, Lindholm, DK-4771 Kalvehave, , 
      Denmark1.
FAU - Oleksiewicz, Martin B
AU  - Oleksiewicz MB
AD  - Danish Veterinary Institute for Virus Research, Lindholm, DK-4771 Kalvehave, , 
      Denmark1.
FAU - Botner, Anette
AU  - Botner A
AD  - Danish Veterinary Institute for Virus Research, Lindholm, DK-4771 Kalvehave, , 
      Denmark1.
LA  - eng
SI  - GENBANK/AF315699
SI  - GENBANK/AF315700
SI  - GENBANK/AF315701
SI  - GENBANK/AF315702
SI  - GENBANK/AF315703
SI  - GENBANK/AF315704
SI  - GENBANK/AF315705
SI  - GENBANK/AF315706
SI  - GENBANK/AF315707
SI  - GENBANK/AF315708
SI  - GENBANK/AF315709
SI  - GENBANK/AF315710
SI  - GENBANK/AF315711
SI  - GENBANK/AY034879
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA, Complementary/genetics
MH  - Molecular Sequence Data
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome virus/classification/*genetics
MH  - RNA, Viral/*genetics
MH  - *Recombination, Genetic
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Swine
EDAT- 2001/10/17 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/17 10:00
PHST- 2001/10/17 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/17 10:00 [entrez]
AID - 10.1099/0022-1317-82-11-2615 [doi]
PST - ppublish
SO  - J Gen Virol. 2001 Nov;82(Pt 11):2615-2620. doi: 10.1099/0022-1317-82-11-2615.