PMID- 11603815
OWN - NLM
STAT- MEDLINE
DCOM- 20020312
LR  - 20190812
IS  - 0001-6322 (Print)
IS  - 0001-6322 (Linking)
VI  - 102
IP  - 4
DP  - 2001 Oct
TI  - CCR2A and CCR2B, the two isoforms of the monocyte chemoattractant protein-1 
      receptor are up-regulated and expressed by different cell subsets in idiopathic 
      inflammatory myopathies.
PG  - 385-92
AB  - The idiopathic inflammatory myopathies (IIM), including dermatomyositis (DM), 
      polymyosititis (PM) and inclusion body myositis (IBM), are a group of autoimmune 
      diseases characterized by chronic lymphocytic and macrophagic infiltration in 
      muscle. The mechanism for recruitment of these cells probably involves 
      chemokines. We have previously reported that monocyte chemoattractant protein-1 
      (MCP-1), a beta chemokine, seems to play a major role in mononuclear cell 
      recruitment especially in DM. Here we have investigated the distribution of the 
      main MCP-1 receptors CCR2A and CCR2B in IIM by polymerase chain reaction (PCR), 
      immunohistochemistry and in situ hybridization. We have shown by reverse 
      transcription-PCR that both CCR2A and CCR2B were expressed at low level in normal 
      muscle and that CCR2A was up-regulated in IIM (P=0.02) and was higher in PM and 
      IBM than in DM (P=0.04). By immunohistochemistry and in situ hybridization we 
      have observed that CCR2 isoforms were expressed by different cell subsets in both 
      normal and IIM muscle. CCR2A was expressed in vessel walls and by some 
      mononuclear cells, especially in cells involved in partial invasion in PM and 
      IBM. CCR2B expression was observed in all satellite cells, in the muscular domain 
      of neuromuscular junctions and in some regenerative fibers of IIM, but not in 
      inflammatory exudates. In conclusion, the present study highlights the major role 
      played by MCP-1 and its counter-receptor CCR2 in the pathophysiology of IIM, and 
      shows that the CCR2 receptors are cell specific. The variation of the total 
      amount of CCR2A and its local distribution according to the type of IIM might be 
      a new path towards the understanding of the constitution of mononuclear 
      infiltrates in IIM.
FAU - Bartoli, C
AU  - Bartoli C
AD  - Laboratoire de Biopathologie Nerveuse et Musculaire, Faculte de medecine, IBDM 
      Marseille, France.
FAU - Civatte, M
AU  - Civatte M
FAU - Pellissier, J F
AU  - Pellissier JF
FAU - Figarella-Branger, D
AU  - Figarella-Branger D
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Acta Neuropathol
JT  - Acta neuropathologica
JID - 0412041
RN  - 0 (CCR2 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Dermatomyositis/pathology/*physiopathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Isomerism
MH  - Male
MH  - Middle Aged
MH  - Muscle, Skeletal/chemistry/pathology
MH  - Myositis, Inclusion Body/pathology/*physiopathology
MH  - RNA, Messenger/analysis
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/analysis/chemistry/*genetics
MH  - Up-Regulation/physiology
EDAT- 2001/10/18 10:00
MHDA- 2002/03/13 10:01
CRDT- 2001/10/18 10:00
PHST- 2001/10/18 10:00 [pubmed]
PHST- 2002/03/13 10:01 [medline]
PHST- 2001/10/18 10:00 [entrez]
AID - 10.1007/s004010100394 [doi]
PST - ppublish
SO  - Acta Neuropathol. 2001 Oct;102(4):385-92. doi: 10.1007/s004010100394.