PMID- 11604067
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20190916
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 156
IP  - 5 Pt 1
DP  - 2001 Nov
TI  - Identification of KIN (KIN17), a human gene encoding a nuclear DNA-binding 
      protein, as a novel component of the TP53-independent response to ionizing 
      radiation.
PG  - 535-44
AB  - Ionizing radiation elicits a genetic response in human cells that allows cell 
      survival. The human KIN (also known as KIN17) gene encodes a 45-kDa nuclear 
      DNA-binding protein that participates in the response to UVC radiation and is 
      immunologically related to the bacterial RecA protein. We report for the first 
      time that ionizing radiation and bleomycin, a radiomimetic drug, which produce 
      single- and double-strand breaks, increased expression of KIN in human cells 
      established from tumors, including MeWo melanoma, MCF7 breast adenocarcinoma, and 
      ATM+ GM3657 lymphoblast cells. KIN expression increased rapidly in a 
      dose-dependent manner after irradiation. Under the same conditions, several genes 
      controlled by TP53 were induced with kinetics similar to that of KIN. Using the 
      CDKN1A gene as a marker of TP53 responsiveness, we analyzed the up-regulation of 
      KIN and showed that is independent of the status of TP53 and ATM. In contrast, 
      the presence of a dominant mutant for activating transcription factor 2 (ATF2) 
      completely abolished the up-regulation of KIN. Our results suggest a role for 
      ATF2 in the TP53-independent increase in KIN expression after gamma irradiation.
FAU - Masson, C
AU  - Masson C
AD  - Laboratoire de Genetique de la Radiosensibilite, Direction des Sciences du 
      Vivant, Centre d'Etudes de Fontenay-aux-Roses, CEA, 60-68, Avenue du 
      General-Leclerc, B.P. no. 6, 92265 Fontenay-aux-Roses Cedex, France.
FAU - Menaa, F
AU  - Menaa F
FAU - Pinon-Lataillade, G
AU  - Pinon-Lataillade G
FAU - Frobert, Y
AU  - Frobert Y
FAU - Radicella, J P
AU  - Radicella JP
FAU - Angulo, J F
AU  - Angulo JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (ATF2 protein, human)
RN  - 0 (Activating Transcription Factor 2)
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Cyclins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KIN protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 11056-06-7 (Bleomycin)
SB  - IM
MH  - Activating Transcription Factor 2
MH  - Bleomycin/pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/physiology
MH  - Cyclin-Dependent Kinase Inhibitor p21
MH  - Cyclins/genetics
MH  - DNA Damage
MH  - DNA-Binding Proteins/*genetics
MH  - Gamma Rays
MH  - Gene Expression Regulation, Neoplastic/*radiation effects
MH  - Humans
MH  - *Nuclear Proteins
MH  - RNA-Binding Proteins
MH  - Transcription Factors/physiology
MH  - Tumor Cells, Cultured
MH  - Tumor Suppressor Protein p53/*physiology
EDAT- 2001/10/18 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/18 10:00
PHST- 2001/10/18 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/18 10:00 [entrez]
AID - 10.1667/0033-7587(2001)156[0535:iokkah]2.0.co;2 [doi]
PST - ppublish
SO  - Radiat Res. 2001 Nov;156(5 Pt 1):535-44. doi: 
      10.1667/0033-7587(2001)156[0535:iokkah]2.0.co;2.