PMID- 11604263
OWN - NLM
STAT- MEDLINE
DCOM- 20011231
LR  - 20190910
IS  - 0378-5173 (Print)
IS  - 0378-5173 (Linking)
VI  - 229
IP  - 1-2
DP  - 2001 Oct 23
TI  - Microencapsulation of dissociable human growth hormone aggregates within 
      poly(D,L-lactic-co-glycolic acid) microparticles for sustained release.
PG  - 107-16
AB  - For the sustained release formulation of recombinant human growth hormone (rhGH), 
      dissociable rhGH aggregates were microencapsulated within 
      poly(D,L-lactic-co-glycolic acid) (PLGA) microparticles. rhGH aggregates were 
      first produced by adding a small volume of aqueous rhGH solution into a partially 
      water miscible organic solvent phase (ethyl acetate, EtAc) containing PLGA. These 
      rhGH aggregates were then microencapsulated within PLGA polymer phase by 
      extracting EtAc into an aqueous phase pre-saturated with EtAc. Release profiles 
      of rhGH from these microparticles were greatly affected by changing the volume of 
      incubation medium. The released rhGH species consisted of mostly monomeric form 
      having a correct conformation. This study reveals that sustained rhGH release 
      could be achieved by microencapsulating reversibly dissociable protein aggregates 
      within biodegradable polymers.
FAU - Kim, H K
AU  - Kim HK
AD  - Department of Biological Sciences, Korea Advanced Institute of Science and 
      Technology, 373-1 Kusong-dong Yusong-gu, 305-701, Taejon, South Korea.
FAU - Park, T G
AU  - Park TG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Int J Pharm
JT  - International journal of pharmaceutics
JID - 7804127
RN  - 0 (Capsules)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Polymers)
RN  - 0 (Solvents)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Capsules
MH  - Chromatography, Gel
MH  - Circular Dichroism
MH  - Delayed-Action Preparations
MH  - Drug Compounding
MH  - Drug Delivery Systems
MH  - Growth Hormone/*administration & dosage
MH  - Humans
MH  - Lactic Acid
MH  - Microscopy, Electron, Scanning
MH  - Particle Size
MH  - Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers
MH  - Solvents
EDAT- 2001/10/18 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/18 10:00
PHST- 2001/10/18 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/18 10:00 [entrez]
AID - S0378517301008523 [pii]
AID - 10.1016/s0378-5173(01)00852-3 [doi]
PST - ppublish
SO  - Int J Pharm. 2001 Oct 23;229(1-2):107-16. doi: 10.1016/s0378-5173(01)00852-3.