PMID- 11606221
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20190822
IS  - 0166-6851 (Print)
IS  - 0166-6851 (Linking)
VI  - 117
IP  - 2
DP  - 2001 Oct
TI  - Calcium regulation in the intraerythrocytic malaria parasite Plasmodium 
      falciparum.
PG  - 121-8
AB  - The regulation of intracellular Ca(2+) in the intraerythrocytic form of the human 
      malaria parasite, Plasmodium falciparum, was investigated using parasites 
      'isolated' from their host cells by saponin-permeabilisation of the erythrocyte 
      membrane. The isolated parasites maintained tight control over their resting 
      cytosolic Ca(2+) concentration which ranged from approximately 100 nM in the 
      absence of extracellular Ca(2+) to approximately 700 nM in the presence of 1 mM 
      extracellular Ca(2+). The parasite has two functionally discrete intracellular 
      Ca(2+) stores. One is an 'endoplasmic reticulum (ER)-like' store, the other an 
      'acidic store'. The ER-like store was discharged by cyclopiazonic acid (CPA), an 
      inhibitor of sarco/endoplasmic reticulum Ca(2+)-ATPases (SERCAs) of animal and 
      plant cells, but not by thapsigargin (TG), a more specific inhibitor of SERCAs of 
      animal cells. The acidic store was discharged by nigericin and by NH(4)(+). The 
      amount of Ca(2+) in the ER-like store increased with increasing extracellular 
      Ca(2+) concentration, whereas the amount of Ca(2+) in the acidic store did not. 
      Ca(2+) released from the ER-like store by CPA was cleared from the parasite 
      cytosol by uptake into the acidic store (over a range of extracellular Ca(2+) 
      concentrations), consistent with the acidic store serving as a Ca(2+) reservoir 
      within the intracellular parasite.
FAU - Alleva, L M
AU  - Alleva LM
AD  - School of Biochemistry and Molecular Biology, Faculty of Science, The Australian 
      National University, Canberra, ACT 0200, Australia.
FAU - Kirk, K
AU  - Kirk K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Culture Media)
RN  - 0 (Indoles)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 67526-95-8 (Thapsigargin)
RN  - RRU6GY95IS (Nigericin)
RN  - SY7Q814VUP (Calcium)
RN  - X9TLY4580Z (cyclopiazonic acid)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Culture Media
MH  - Erythrocytes/*parasitology
MH  - *Homeostasis
MH  - Hydrogen-Ion Concentration
MH  - Indoles/pharmacology
MH  - Malaria, Falciparum/parasitology
MH  - Nigericin/pharmacology
MH  - Plasmodium falciparum/*physiology
MH  - Quaternary Ammonium Compounds/pharmacology
MH  - Thapsigargin/pharmacology
EDAT- 2001/10/19 10:00
MHDA- 2002/02/01 10:01
CRDT- 2001/10/19 10:00
PHST- 2001/10/19 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2001/10/19 10:00 [entrez]
AID - S0166685101003383 [pii]
AID - 10.1016/s0166-6851(01)00338-3 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 2001 Oct;117(2):121-8. doi: 10.1016/s0166-6851(01)00338-3.