PMID- 11606634
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20240420
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 21
IP  - 21
DP  - 2001 Nov 1
TI  - Sodium channel mRNAs at the neuromuscular junction: distinct patterns of 
      accumulation and effects of muscle activity.
PG  - 8456-63
AB  - Voltage-gated sodium channels (VGSCs) are highly concentrated at the 
      neuromuscular junction (NMJ) in mammalian skeletal muscle. Here we test the 
      hypothesis that local upregulation of mRNA contributes to this accumulation. We 
      designed radiolabeled antisense RNA probes, specific for the "adult" Na(V)1.4 and 
      "fetal" Na(V)1.5 isoforms of VGSC in mammalian skeletal muscle, and used them in 
      in situ hybridization studies of rat soleus muscles. Na(V)1.4 mRNA is present 
      throughout normal adult muscles but is highly concentrated at the NMJ, in which 
      the amount per myonucleus is more than eightfold greater than away from the NMJ. 
      Na(V)1.5 mRNA is undetectable in innervated muscles but is dramatically 
      upregulated by denervation. In muscles denervated for 1 week, both Na(V)1.4 and 
      Na(V)1.5 mRNAs are present throughout the muscle, and both are concentrated at 
      the NMJ. No Na(V)1.5 mRNA was detectable in denervated muscles stimulated 
      electrically for 1 week in vivo. Neither denervation nor stimulation had any 
      significant effect on the level or distribution of Na(V)1.4 mRNA. We conclude 
      that factors, probably derived from the nerve, lead to the increased 
      concentration of VGSC mRNAs at the NMJ. In addition, the expression of Na(V)1.5 
      mRNA is downregulated by muscle activity, both at the NMJ and away from it.
FAU - Awad, S S
AU  - Awad SS
AD  - Department of Neuroscience, University of Newcastle upon Tyne, NE2 4HH, Newcastle 
      upon Tyne, United Kingdom. s.s.awad@ncl.ac.uk
FAU - Lightowlers, R N
AU  - Lightowlers RN
FAU - Young, C
AU  - Young C
FAU - Chrzanowska-Lightowlers, Z M
AU  - Chrzanowska-Lightowlers ZM
FAU - Lomo, T
AU  - Lomo T
FAU - Slater, C R
AU  - Slater CR
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Oligoribonucleotides, Antisense)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sodium Channels)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Electric Stimulation/methods
MH  - Female
MH  - In Situ Hybridization
MH  - Muscle Contraction/physiology
MH  - Muscle Denervation
MH  - Muscle, Skeletal/innervation/*physiology
MH  - Neuromuscular Junction/*metabolism
MH  - Oligoribonucleotides, Antisense/genetics/metabolism
MH  - Polymerase Chain Reaction
MH  - Protein Isoforms/genetics/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Sodium Channels/genetics/*metabolism
MH  - Up-Regulation
PMC - PMC6762790
EDAT- 2001/10/19 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/05/01
CRDT- 2001/10/19 10:00
PHST- 2001/10/19 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/19 10:00 [entrez]
PHST- 2002/05/01 00:00 [pmc-release]
AID - 21/21/8456 [pii]
AID - 5762 [pii]
AID - 10.1523/JNEUROSCI.21-21-08456.2001 [doi]
PST - ppublish
SO  - J Neurosci. 2001 Nov 1;21(21):8456-63. doi: 10.1523/JNEUROSCI.21-21-08456.2001.