PMID- 11641259
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20120215
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 15
IP  - 13
DP  - 2001 Nov
TI  - Monovalent fusion proteins of IgE mimotopes are safe for therapy of type I 
      allergy.
PG  - 2524-6
AB  - By screening phage display random peptide libraries with purified immunoglobulin 
      E (IgE) from birch pollen-allergic patients, we previously defined peptides 
      mimicking natural IgE epitopes (mimotopes) of the major birch pollen allergen Bet 
      v 1. The present study aimed to define a monovalent carrier for the IgE mimotopes 
      to induce protective antibodies directed to the IgE epitopes, suitable for 
      mimotope-specific therapy. We expressed the selected mimotopes as fusion proteins 
      together with streptococcal albumin binding protein (ABP). The fusion proteins 
      were recognized specifically by anti-Bet v 1 human IgE, which demonstrated that 
      the mimotopes fused to ABP resemble the natural IgE epitope. Bet v 1-specific IgG 
      was induced by immunization of BALB/c mice with fusion proteins. These IgG 
      antibodies could inhibit IgE binding to Bet v 1. Skin testing of Bet v 1 allergic 
      mice showed that the ABP mimotope constructs did not elicit type I skin 
      reactions, although they possess IgE binding structures. Our data suggest that 
      IgE mimotopes are safe for epitope-specific immunotherapy of sensitized 
      individuals, when presented in a monovalent form. Therefore, ABP-fused mimotopes 
      are promising candidates for a new type of immunotherapy based on the precise 
      induction of blocking antibodies.
FAU - Ganglberger, E
AU  - Ganglberger E
AD  - Department of Pathophysiology, University of Vienna, A-1090 Vienna, Austria.
FAU - Barbara Sponer
AU  - Barbara Sponer
FAU - Scholl, I
AU  - Scholl I
FAU - Wiedermann, U
AU  - Wiedermann U
FAU - Baumann, S
AU  - Baumann S
FAU - Hafner, C
AU  - Hafner C
FAU - Breiteneder, H
AU  - Breiteneder H
FAU - Suter, M
AU  - Suter M
FAU - Boltz-Nitulescu, G
AU  - Boltz-Nitulescu G
FAU - Scheiner, O
AU  - Scheiner O
FAU - Jensen-Jarolim, E
AU  - Jensen-Jarolim E
LA  - eng
PT  - Journal Article
DEP - 20010917
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Allergens)
RN  - 0 (Antibodies)
RN  - 0 (Antigens, Plant)
RN  - 0 (Contractile Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (Profilins)
RN  - 0 (Receptors, Albumin)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 126161-14-6 (Bet v 1 allergen, Betula)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - *Allergens
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies/blood
MH  - Antibody Formation
MH  - Antigens, Plant
MH  - *Contractile Proteins
MH  - Epitopes/genetics/*immunology
MH  - Humans
MH  - Hypersensitivity/*immunology
MH  - Immunoglobulin E/genetics/*immunology
MH  - Immunoglobulin G/blood
MH  - Immunotherapy
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microfilament Proteins/genetics/immunology
MH  - Molecular Mimicry/genetics/*immunology
MH  - Plant Proteins/immunology
MH  - Plasmids/genetics
MH  - Profilins
MH  - Receptors, Albumin/genetics
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Skin Tests
EDAT- 2001/10/20 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/20 10:00
PHST- 2001/10/20 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/20 10:00 [entrez]
AID - 00-0888fje [pii]
AID - 10.1096/fj.00-0888fje [doi]
PST - ppublish
SO  - FASEB J. 2001 Nov;15(13):2524-6. doi: 10.1096/fj.00-0888fje. Epub 2001 Sep 17.