PMID- 11641520
OWN - NLM
STAT- MEDLINE
DCOM- 20020103
LR  - 20131121
IS  - 1011-8934 (Print)
IS  - 1598-6357 (Electronic)
IS  - 1011-8934 (Linking)
VI  - 16
IP  - 5
DP  - 2001 Oct
TI  - Varying effects of intravenous immunoglobulin on mononuclear cell proliferation 
      in vitro.
PG  - 544-8
AB  - Intravenous immunoglobulin (IVIG) is being increasingly used to treat numerous 
      immune-mediated diseases. However, there is a paucity of knowledge on the 
      specific mode of action of IVIG in vivo. In this study, the in vitro effects of 
      IVIG on peripheral blood mononuclear cell (PBMC) proliferation using 
      phytohemagglutinin (PHA), anti-CD3 monoclonal antibody (MAb), phorbol myristate 
      acetate (PMA), or purified protein derivatives (PPD) have been analyzed. The 
      PBMCs were obtained from more than 10 individual donors. In all cases, IVIG 
      almost completely inhibited PBMC proliferation at concentration above 20 mg/mL 
      except when used in conjunction with PMA. PHA-induced proliferation of PBMCs at 
      concentrations ranging from 1 to 15 mg/mL did not show significant differences. 
      Anti-CD3 MAb-induced proliferation showed dose-dependent inhibition at 
      concentrations ranging from 1 to 10 mg/mL. Interestingly, PMA-induced 
      proliferation of PBMCs showed a dose-dependent increase at the same concentration 
      range. PPD-induced proliferation of PBMC at concentrations ranging from 1 to 10 
      mg/mL did not show any statistically significant differences. These results 
      suggest that high dose IVIG may be necessary to immune modulation in vivo and 
      IVIG has various effects on PBMCs proliferation in limited concentration in 
      vitro.
FAU - Lee, K Y
AU  - Lee KY
AD  - Department of Pediatrics, College of Medicine, The Catholic University of Korea, 
      Seoul, Korea.
FAU - Koh, D K
AU  - Koh DK
FAU - Lee, J S
AU  - Lee JS
FAU - Whang, K T
AU  - Whang KT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Korean Med Sci
JT  - Journal of Korean medical science
JID - 8703518
RN  - 0 (Immunoglobulins, Intravenous)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Immunoglobulins, Intravenous/*pharmacology
MH  - Leukocytes, Mononuclear/*drug effects/physiology
MH  - Tetradecanoylphorbol Acetate/pharmacology
PMC - PMC3057596
EDAT- 2001/10/20 10:00
MHDA- 2002/01/05 10:01
PMCR- 2001/10/01
CRDT- 2001/10/20 10:00
PHST- 2001/10/20 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/20 10:00 [entrez]
PHST- 2001/10/01 00:00 [pmc-release]
AID - 200110544 [pii]
AID - 10.3346/jkms.2001.16.5.544 [doi]
PST - ppublish
SO  - J Korean Med Sci. 2001 Oct;16(5):544-8. doi: 10.3346/jkms.2001.16.5.544.