PMID- 11641604
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20171101
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 126
IP  - 1
DP  - 2001 Sep
TI  - Hypoallergenic variants of the Parietaria judaica major allergen Par j 1: a 
      member of the non-specific lipid transfer protein plant family.
PG  - 32-40
AB  - BACKGROUND: Par j 1 represents a major allergenic component of Parietaria judaica 
      (Pj) pollen, since it is able to induce an immunoglobulin E (IgE) response in 95% 
      of Pj-allergic patients. It belongs to the non-specific lipid transfer protein 
      family, sharing with them a common three-dimensional structure. METHODS: 
      Disulphide bond variants of the recombinant Par j 1 (rPar j 1) allergen were 
      generated by site-directed mutagenesis, and the immunological activity of rPar j 
      1 and its conformational mutants was compared with the use of the skin prick test 
      (SPT). The ability to bind IgE antibodies was evaluated by Western blot, ELISA 
      and ELISA inhibition. T cell reactivity was measured by peripheral blood 
      mononuclear cell proliferation assay. RESULTS: The disruption of Cys14-Cys29 and 
      Cys30-Cys75 bridging (PjA mutant) caused the loss of the majority of specific 
      IgE-binding activity. Additional disruption of the Cys4-Cys52 bridge (PjC mutant) 
      and the latter Cys50-Cys91 bridge (PjD mutant) led to the abolition of 
      IgE-binding activity. On the SPT, PjB (lacking the Cys4-Cys52 and Cys50-Cys91 
      bridges) was still capable of triggering a type I hypersensitive reaction in 9 
      out of 10 patients, and PjA in 3 out of 10 patients, while PjC and PjD did not 
      show any SPT reactivity. All the mutants preserved their T cell reactivity. 
      CONCLUSION: Recombinant hypoallergenic variants of the rPar j 1 allergen 
      described herein may represent a useful tool for improved immunotherapy.
CI  - Copyright 2001 S. Karger AG, Basel
FAU - Bonura, A
AU  - Bonura A
AD  - Istituto di Biologia dello Sviluppo del Consiglio Nazionale delle Ricerche, 
      Palermo, Italia.
FAU - Amoroso, S
AU  - Amoroso S
FAU - Locorotondo, G
AU  - Locorotondo G
FAU - Di Felice, G
AU  - Di Felice G
FAU - Tinghino, R
AU  - Tinghino R
FAU - Geraci, D
AU  - Geraci D
FAU - Colombo, P
AU  - Colombo P
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Plant)
RN  - 0 (Disulfides)
RN  - 0 (Glycoproteins)
RN  - 0 (Par j I protein, Parietaria judaica)
RN  - 0 (Plant Proteins)
RN  - 0 (lipid transfer proteins, plant)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/*genetics
MH  - Animals
MH  - Antigens, Plant
MH  - Base Sequence
MH  - Carrier Proteins/chemistry/*genetics/*immunology
MH  - DNA, Plant/genetics
MH  - Desensitization, Immunologic
MH  - Disulfides/chemistry
MH  - Genetic Variation
MH  - Glycoproteins/chemistry/*genetics
MH  - Humans
MH  - Hypersensitivity, Immediate/immunology/therapy
MH  - Immunoglobulin E/metabolism
MH  - In Vitro Techniques
MH  - Lymphocyte Activation
MH  - Plant Proteins/chemistry/*genetics
MH  - Rabbits
MH  - T-Lymphocytes/immunology
MH  - Urticaceae/genetics/immunology
EDAT- 2001/10/20 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/20 10:00
PHST- 2001/10/20 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/20 10:00 [entrez]
AID - 49492 [pii]
AID - 10.1159/000049492 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2001 Sep;126(1):32-40. doi: 10.1159/000049492.