PMID- 11642005
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20041117
IS  - 0004-4172 (Print)
IS  - 0004-4172 (Linking)
VI  - 51
IP  - 9
DP  - 2001 Sep
TI  - Effects of phycocyanin extract on tumor necrosis factor-alpha and nitrite levels 
      in serum of mice treated with endotoxin.
PG  - 733-6
AB  - Phycocyanin is a biliprotein which exerts antioxidative and anti-inflammatory 
      effects in various in vivo and in vitro experimental models. In this study 
      phycocyanin effects on tumor necrosis factor-alpha (TNF alpha) and nitrite levels 
      in serum of mice treated with lipopolysaccharide (LPS) was examined. TNF alpha 
      was measured by cytotoxicity on L-929 cells and nitrite by the Griess reaction, 
      after reduction of all nitrates to nitrites by nitrate reductase, 1 h after LPS 
      injection (0.5 mg/kg i.p.) there was a significant increase in TNF alpha levels 
      in mouse serum. Phycocyanin (50-300 mg/kg p.o.), administered 1 h before LPS, 
      reduced dose-dependently the TNF alpha concentration in serum. After 18 h, LPS 
      (30 mg/kg i.p.) also induced a substantial increase in serum nitrite levels, 
      which were reduced dose-dependently by phycocyanin pretreatment (100-300 mg/kg 
      p.o.). The results indicate that phycocyanin exerts inhibitory effects on TNF 
      alpha and NO production which might be ascribed to the antioxidative properties 
      of the biliprotein.
FAU - Romay, C
AU  - Romay C
AD  - Department of Pharmacology, National Center for Scientific Research, Havana, 
      Cuba. ozono@infomed.sld.cu
FAU - Delgado, R
AU  - Delgado R
FAU - Remirez, D
AU  - Remirez D
FAU - Gonzalez, R
AU  - Gonzalez R
FAU - Rojas, A
AU  - Rojas A
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Arzneimittelforschung
JT  - Arzneimittel-Forschung
JID - 0372660
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nitrites)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11016-15-2 (Phycocyanin)
RN  - 67924-63-4 (endotoxin, Escherichia coli)
SB  - IM
MH  - Animals
MH  - Endotoxins/*pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Mice
MH  - Nitrites/*blood
MH  - Phycocyanin/*pharmacology
MH  - Tumor Necrosis Factor-alpha/*metabolism
EDAT- 2001/10/20 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/20 10:00
PHST- 2001/10/20 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/20 10:00 [entrez]
AID - 10.1055/s-0031-1300107 [doi]
PST - ppublish
SO  - Arzneimittelforschung. 2001 Sep;51(9):733-6. doi: 10.1055/s-0031-1300107.