PMID- 11669471
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 69
IP  - 19
DP  - 2001 Sep 28
TI  - Cytokine and nitric oxide release by J774A.1 macrophages is not regulated by 
      estradiol.
PG  - 2287-94
AB  - Estradiol is able to regulate the release of inflammatory mediators by 
      macrophages; however, the presence, extent, and direction of this modulation 
      varies with species, tissue of origin, and cell culture conditions. This study 
      examines the effects of 17-beta-estradiol (E2) on the release of inflammatory 
      mediators by the J774A.1 mouse macrophage cell line. For experiments, cells were 
      plated in phenol red-free DMEM containing 5% charcoal-dextran stripped calf 
      serum. Western analysis showed that J774A.1 cells contain the estrogen receptor 
      alpha (ER alpha) protein. We found that physiological and pharmacological levels 
      of E2 (10(-12) M-10(-6) M) have no effect on the release of nitric oxide (NO), 
      tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), or monocyte 
      chemoattractant protein-1 (MCP-1). This suggests that J774A.1 cells grown under 
      these culture conditions would be useful for the investigation of 
      non-estrogen-dependent mechanisms by which certain endocrine disruptors may 
      affect their targets in macrophages.
FAU - Woodfork, K A
AU  - Woodfork KA
AD  - Pathology and Physiology Research Branch, Health Effects Laboratory Division, 
      National Institute of Occupational Safety and Health, Morgantown, WV 26505, USA. 
      kwoodfork@hsc.wvu.edu
FAU - Schuller, K C
AU  - Schuller KC
FAU - Huffman, L J
AU  - Huffman LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/*metabolism
MH  - Estradiol/*pharmacology
MH  - Macrophages/*metabolism
MH  - Mice
MH  - Nitric Oxide/*metabolism
EDAT- 2001/10/24 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/24 10:00
PHST- 2001/10/24 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/24 10:00 [entrez]
AID - S0024-3205(01)01304-2 [pii]
AID - 10.1016/s0024-3205(01)01304-2 [doi]
PST - ppublish
SO  - Life Sci. 2001 Sep 28;69(19):2287-94. doi: 10.1016/s0024-3205(01)01304-2.