PMID- 11672768
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 130
IP  - 1
DP  - 2001 Oct 1
TI  - Comparative genomic hybridization analysis identifies gains of 1p35 approximately 
      p36 and chromosome 19 in osteosarcoma.
PG  - 14-21
AB  - Osteosarcomas (OS) are aggressive tumors of the bone and often have a poor 
      prognosis. Conventional cytogenetic analyses of OS have revealed highly complex 
      karyotypes, with numerous abnormalities. In this study, we analyzed 18 untreated 
      OS tumors from 17 patients of the younger incidence age group by comparative 
      genomic hybridization (CGH), 4 tumors by spectral karyotyping (SKY) and 
      fluorescence in situ hybridization (FISH). Comparative genomic hybridization 
      identified frequent copy number changes of the chromosomal region 1p (10/17) and 
      gain of part or all of chromosome 19(8/17). In addition gains were observed at 
      5p(3/17), 8q(3/17), 16p(3/17), and 17p(5/17); and losses at chromosomes 2q(3/17), 
      10(4/17) and 13(3/17). High level gains were detected in the 8q23 approximately 
      q24 region in two tumors as well as at 17p in one primary and a metastatic tumor. 
      Minimal regions of gain were present at 1p35 approximately p36.3 (8/17); 5p14 
      approximately p15.2 (3/17), and 8q22 approximately q24.3 (3/17). SKY analysis 
      demonstrated that OS has a complex pattern of clonal and non-clonal 
      rearrangements and helped confirm the structural basis for the imbalances 
      detected by CGH. Spectral karyotyping confirmed an overall pattern of chromosomal 
      gain affecting 1p in all four tumors. Fluorescence in situ hybridization analysis 
      from these tumors confirmed the gain of the 1p36 region in 2 tumors as determined 
      by CGH analysis as well as the amplification of 8q.
FAU - Zielenska, M
AU  - Zielenska M
AD  - Department of Laboratory Medicine and Pathobiology, University of Toronto, 
      Princess Margaret Hospital, Toronto, Ontario, Canada.
FAU - Bayani, J
AU  - Bayani J
FAU - Pandita, A
AU  - Pandita A
FAU - Toledo, S
AU  - Toledo S
FAU - Marrano, P
AU  - Marrano P
FAU - Andrade, J
AU  - Andrade J
FAU - Petrilli, A
AU  - Petrilli A
FAU - Thorner, P
AU  - Thorner P
FAU - Sorensen, P
AU  - Sorensen P
FAU - Squire, J A
AU  - Squire JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Bone Neoplasms/*genetics
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 19
MH  - Genome
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Nucleic Acid Hybridization
MH  - Osteosarcoma/*genetics
EDAT- 2001/10/24 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/24 10:00
PHST- 2001/10/24 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/24 10:00 [entrez]
AID - S0165-4608(01)00461-7 [pii]
AID - 10.1016/s0165-4608(01)00461-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Oct 1;130(1):14-21. doi: 
      10.1016/s0165-4608(01)00461-7.