PMID- 11673622
OWN - NLM
STAT- MEDLINE
DCOM- 20020314
LR  - 20190513
IS  - 0032-0781 (Print)
IS  - 0032-0781 (Linking)
VI  - 42
IP  - 10
DP  - 2001 Oct
TI  - RNA binding-proteins interact specifically with the Arabidopsis chloroplast psbA 
      mRNA 5' untranslated region in a redox-dependent manner.
PG  - 1071-8
AB  - The 5' untranslated region (5'UTR) of the psbA mRNA (psbA encodes the PSII 
      reaction center protein, D1) is a key site for RNA-protein interactions in the 
      post-transcriptional regulation of gene expression. In this study, we mapped the 
      major psbA mRNA 5'-terminus at -77 nt, and two minor termini clusters centered at 
      -48 and -64 nt, upstream from the psbA translational start codon of Arabidopsis 
      thaliana. RNA mobility shift, RNase protection and UV-crosslinking assays were 
      used to characterize the interaction of chloroplast proteins with the RNA 5'UTR. 
      RNA-protein interactions depended upon a thermolabile secondary structure and 
      specific sequences in a 35 nt region of the 5'UTR, which were 80% conserved with 
      the psbA 5'UTRs from five other plants. Major and minor proteins of 43- and 
      30-kDa, respectively, were detected by UV-crosslinking to RNA. Oxidizing 
      conditions abolished the association of the proteins with the 5'UTR, while 
      RNA-binding activity was recovered upon incubation with a reductant. Based on 
      these findings, we hypothesize that post-transcriptional regulation of psbA gene 
      expression in chloroplasts of vascular plants involves redox-dependent 
      interactions between specific sequences in the 5'UTR and 43- and 30-kDa 
      RNA-binding proteins.
FAU - Shen, Y
AU  - Shen Y
AD  - Department of Molecular Biosciences and Biosystems Engineering, University of 
      Hawaii, 1955 East-West Road, Honolulu, HI 96822 USA.
FAU - Danon, A
AU  - Danon A
FAU - Christopher, D A
AU  - Christopher DA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Photosynthetic Reaction Center Complex Proteins)
RN  - 0 (Photosystem II Protein Complex)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - *5' Untranslated Regions
MH  - Arabidopsis/*genetics
MH  - Base Sequence
MH  - Chloroplasts/*genetics
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - Oxidation-Reduction
MH  - Photosynthetic Reaction Center Complex Proteins/*genetics
MH  - Photosystem II Protein Complex
MH  - Protein Binding
MH  - RNA, Messenger/chemistry/*genetics
MH  - RNA-Binding Proteins/*metabolism
MH  - Sequence Homology, Nucleic Acid
EDAT- 2001/10/24 10:00
MHDA- 2002/03/15 10:01
CRDT- 2001/10/24 10:00
PHST- 2001/10/24 10:00 [pubmed]
PHST- 2002/03/15 10:01 [medline]
PHST- 2001/10/24 10:00 [entrez]
AID - 10.1093/pcp/pce142 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2001 Oct;42(10):1071-8. doi: 10.1093/pcp/pce142.