PMID- 11675131
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 130
IP  - 2
DP  - 2001 Oct 15
TI  - Cytogenetic and fluorescence in situ hybridization characterization of chromosome 
      8 rearrangements in head and neck squamous cell carcinomas.
PG  - 111-7
AB  - Structural rearrangements of chromosome 8 are frequently encountered in squamous 
      cell carcinomas of the head and neck (HNSCC). These aberrations often affect the 
      centromeric region, resulting in the formation of isochromosome i(8q) and whole 
      arm translocations. Some tumors may display structural rearrangements of 8p23. To 
      characterize further the localization of the breakpoints in such rearrangements, 
      12 HNSCC known to carry pericentromeric rearrangements of chromosome 8 and 8p23 
      abnormalities were investigated with fluorescence in situ hybridization (FISH) by 
      the use of 15 YAC clones spanning 8p23 and 8p11 to 8q11. FISH confirmed that all, 
      except one, aberrations cytogenetically interpreted to be i(8q) were true, 
      monocentric i(8q). Similarly, all whole-arm translocations appeared as centric 
      fusions. It could thus be concluded that the essential outcome of these 
      rearrangements is genomic imbalances and not rearrangement of genes in the 
      pericentromeric region. By the use of five YAC clones mapping to 8p23, different 
      breakpoints at the molecular level were disclosed in cases with cytogenetically 
      identical 8p23 rearrangements. An evaluation of the genomic imbalances detected 
      in the present series revealed that overrepresentation of 8q material was present 
      in 11 of the 12 tumors. The most commonly gained segment was 8q22 approximately 
      qter, found in all cases with 8q overrepresentation. Loss of parts of or the 
      entire 8p was seen in 10 tumors. The smallest overlapping deleted region was 
      localized to the subtelomeric region of 8p.
FAU - Jin, Y
AU  - Jin Y
AD  - Department of Clinical Genetics, University Hospital, S-221 85, Lund, Sweden. 
      yuesheng.jin@klingen.lu.se
FAU - Jin, C
AU  - Jin C
FAU - Wennerberg, J
AU  - Wennerberg J
FAU - Hoglund, M
AU  - Hoglund M
FAU - Mertens, F
AU  - Mertens F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chromosome Aberrations
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 8/*ultrastructure
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Head and Neck Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Isochromosomes
MH  - Male
MH  - Middle Aged
MH  - Models, Genetic
MH  - *Mutation
EDAT- 2001/10/25 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/25 10:00
PHST- 2001/10/25 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/25 10:00 [entrez]
AID - S0165-4608(01)00476-9 [pii]
AID - 10.1016/s0165-4608(01)00476-9 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2001 Oct 15;130(2):111-7. doi: 
      10.1016/s0165-4608(01)00476-9.