PMID- 11676861
OWN - NLM
STAT- MEDLINE
DCOM- 20020111
LR  - 20190513
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 92
IP  - 10
DP  - 2001 Oct
TI  - Cyclin D1 antisense oligonucleotide inhibits cell growth stimulated by epidermal 
      growth factor and induces apoptosis of gastric cancer cells.
PG  - 1102-9
AB  - The cyclin D1 protein is one of the cell cycle regulators required for cell cycle 
      progression through G1 phase to S phase. The cyclin D1-cyclin-dependent kinase 
      (CDK) system is thought to control the cell cycle through mediating extracellular 
      signals from mitogens, such as epidermal growth factor (EGF). In this study, we 
      attempted to examine the therapeutic effect of cyclin D1 antisense 
      oligonucleotides (AS/D1) on cell proliferation and apoptosis of the gastric 
      cancer cell line MKN-74, in the presence and absence of EGF-stimulation. 
      Evaluation of cell survival and DNA synthesis revealed that enhanced cell growth 
      following EGF-stimulation was completely inhibited by a 24 h pre-incubation with 
      100 nM AD/D1. This inhibition was down to 19.3% compared with maximal DNA 
      synthesis after stimulation with 3 nM EGF alone. Western blotting demonstrated 
      that while EGF-stimulation led to cyclin D1 over-expression, AS/D1 inhibited 
      cyclin D1 protein expression. We also demonstrated the induction of apoptosis in 
      MKN-74 cells by AS/D1. In conclusion, EGF-stimulated MKN-74 cell proliferation 
      was inhibited by AS/D1, which could overcome EGF-induced cyclin D1 
      over-expression. AS/D1 also affected cell survival by inducing apoptosis through 
      cell cycle arrest following cyclin D1 depletion. Thus, AS/D1 may be a candidate 
      for use as a novel cancer therapy specifically targeted against the 
      over-expression of cyclin D1 enhanced by EGF in malignant cells.
FAU - Saikawa, Y
AU  - Saikawa Y
AD  - Department of Surgery, Hiratsuka City Hospital, Hiratsuka, Kanagawa 254-0065. 
      saikmimi@aol.com
FAU - Kubota, T
AU  - Kubota T
FAU - Otani, Y
AU  - Otani Y
FAU - Kitajima, M
AU  - Kitajima M
FAU - Modlin, I M
AU  - Modlin IM
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (Oligonucleotides, Antisense)
RN  - 136601-57-5 (Cyclin D1)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Blotting, Western
MH  - Cell Division/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cyclin D1/biosynthesis/*genetics/metabolism
MH  - DNA/biosynthesis
MH  - Dose-Response Relationship, Drug
MH  - Epidermal Growth Factor/*pharmacology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Oligonucleotides, Antisense/genetics/*pharmacology
MH  - Stomach Neoplasms/*pathology
MH  - Time Factors
MH  - Tumor Cells, Cultured
PMC - PMC5926617
EDAT- 2001/10/26 10:00
MHDA- 2002/01/12 10:01
PMCR- 2001/10/01
CRDT- 2001/10/26 10:00
PHST- 2001/10/26 10:00 [pubmed]
PHST- 2002/01/12 10:01 [medline]
PHST- 2001/10/26 10:00 [entrez]
PHST- 2001/10/01 00:00 [pmc-release]
AID - CAE1102 [pii]
AID - 10.1111/j.1349-7006.2001.tb01065.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 2001 Oct;92(10):1102-9. doi: 
      10.1111/j.1349-7006.2001.tb01065.x.