PMID- 11677628
OWN - NLM
STAT- MEDLINE
DCOM- 20011220
LR  - 20220310
IS  - 0022-2844 (Print)
IS  - 0022-2844 (Linking)
VI  - 53
IP  - 6
DP  - 2001 Dec
TI  - Mycobacterium tuberculosis phylogeny reconstruction based on combined numerical 
      analysis with IS1081, IS6110, VNTR, and DR-based spoligotyping suggests the 
      existence of two new phylogeographical clades.
PG  - 680-9
AB  - This paper deals with phylogenetic relationships among a set of 90 clinical 
      strains representative of the worldwide diversity of the Mycobacterium 
      tuberculosis complex (Kremer et al. 1999) using eight independent genetic 
      markers: IS6110, IS1081, the direct repeat (DR) locus, and five variable number 
      of tandem DNA repeat loci (VNTR). In a preliminary experiment, phylogenetic trees 
      based on single markers were constructed that led to the detection of some 
      similarities between the VNTR-based and the spoligotyping-based phylogenetic 
      trees. In the second step, a more global phenetic approach based on pairwise 
      comparison of strains within each typing system was used, followed by 
      calculations of mean genetic distances based on all the eight loci and the use of 
      the neighbor-joining algorithm for tree reconstruction. This analysis confirmed 
      our preliminary observations and suggested the existence of at least two new 
      phylogeographical clades of M. tuberculosis, one defined as the "East 
      African-Indian family" (EA-I), which may find its origin on the African or Asian 
      continents, and the other as the "Latin American and Mediterranean" (LA-M) 
      family. The existence of these two families was also validated by an independent 
      phylogenetic analysis of spoligotyping on a larger set of shared types (n = 252) 
      and further corroborated by VNTR and katG-gyrA results. The potential origin of 
      these families of bacilli is discussed based on cattle domestication and human 
      migration history. In conclusion, the information contained in insertion sequence 
      and repetitive DNAs may serve as a model for the phylogenetic reconstruction of 
      the M. tuberculosis complex.
FAU - Sola, C
AU  - Sola C
AD  - Unite de la Tuberculose et des Mycobacteries, Institut Pasteur de Guadeloupe, 
      Morne Joliviere, BP 484, F-97165 Pointe a Pitre-Cedex, Guadeloupe. 
      csola@pasteur.gp
FAU - Filliol, I
AU  - Filliol I
FAU - Legrand, E
AU  - Legrand E
FAU - Mokrousov, I
AU  - Mokrousov I
FAU - Rastogi, N
AU  - Rastogi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Mol Evol
JT  - Journal of molecular evolution
JID - 0360051
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Bacterial Typing Techniques
MH  - Biological Evolution
MH  - DNA Transposable Elements
MH  - DNA, Bacterial
MH  - *Genetic Markers
MH  - Genetic Variation
MH  - Humans
MH  - Minisatellite Repeats
MH  - Mycobacterium tuberculosis/*classification
MH  - Phylogeny
EDAT- 2001/10/26 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/26 10:00
PHST- 2001/10/26 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/26 10:00 [entrez]
AID - 10.1007/s002390010255 [doi]
PST - ppublish
SO  - J Mol Evol. 2001 Dec;53(6):680-9. doi: 10.1007/s002390010255.