PMID- 11678371
OWN - NLM
STAT- MEDLINE
DCOM- 20020402
LR  - 20190901
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 762
IP  - 2
DP  - 2001 Oct 25
TI  - Electroosmotic properties of microfluidic channels composed of 
      poly(dimethylsiloxane).
PG  - 117-25
AB  - Microfluidic devices fabricated from polymers exhibit great potential in 
      biological analyses. Poly(dimethylsiloxane) (PDMS) has shown promise as a 
      substrate for rapid prototyping of devices. Despite this, disagreement exists in 
      the literature as to the ability of PDMS to support electroosmotic (EO) flow and 
      the stability of that flow over time. We demonstrate that in low ionic strength 
      solutions near neutral in pH. oxidized PDMS had a four-fold greater EO mobility 
      (mu(eo)) compared to native PDMS. The greater mu(eo) was maintained irrespective 
      of whether glass or PDMS was used as a support forming one side of the channel. 
      This enhanced mu(eo) was preserved as long as the channels were filled with an 
      aqueous solution. Upon exposure of the channels to air, the mobility decreased by 
      a factor of two with a half-life of 9 h. The EO properties of the air-exposed, 
      oxidized PDMS were regenerated by exposure to strong base. High ionic strength, 
      neutral in pH buffers compatible with living eukaryotic cells diminished the EO 
      flow in the oxidized PDMS devices to a much greater extent than in the native 
      PDMS devices. For analyses utilizing intact and living cells, oxidation of PDMS 
      may not be an effective strategy to substantially increase the mu(eo).
FAU - Ren, X
AU  - Ren X
AD  - Center for Biomedical Engineering, University of California, Irvine 92697, USA.
FAU - Bachman, M
AU  - Bachman M
FAU - Sims, C
AU  - Sims C
FAU - Li, G P
AU  - Li GP
FAU - Allbritton, N
AU  - Allbritton N
LA  - eng
GR  - CA78858/CA/NCI NIH HHS/United States
GR  - KO8AI01513/AI/NIAID NIH HHS/United States
GR  - RR/CA14892/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Electrophoresis
MH  - Hydrogen-Ion Concentration
MH  - Osmolar Concentration
MH  - Osmosis
MH  - Silicones/*chemistry
MH  - Spectrophotometry, Infrared
EDAT- 2001/10/27 10:00
MHDA- 2002/04/03 10:01
CRDT- 2001/10/27 10:00
PHST- 2001/10/27 10:00 [pubmed]
PHST- 2002/04/03 10:01 [medline]
PHST- 2001/10/27 10:00 [entrez]
AID - 10.1016/s0378-4347(01)00327-9 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 2001 Oct 25;762(2):117-25. doi: 
      10.1016/s0378-4347(01)00327-9.