PMID- 11679356
OWN - NLM
STAT- MEDLINE
DCOM- 20020117
LR  - 20191210
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 67
IP  - 11
DP  - 2001 Nov
TI  - In situ characterization of Nitrospira-like nitrite-oxidizing bacteria active in 
      wastewater treatment plants.
PG  - 5273-84
AB  - Uncultivated Nitrospira-like bacteria in different biofilm and activated-sludge 
      samples were investigated by cultivation-independent molecular approaches. 
      Initially, the phylogenetic affiliation of Nitrospira-like bacteria in a 
      nitrifying biofilm was determined by 16S rRNA gene sequence analysis. 
      Subsequently, a phylogenetic consensus tree of the Nitrospira phylum including 
      all publicly available sequences was constructed. This analysis revealed that the 
      genus Nitrospira consists of at least four distinct sublineages. Based on these 
      data, two 16S rRNA-directed oligonucleotide probes specific for the phylum and 
      genus Nitrospira, respectively, were developed and evaluated for suitability for 
      fluorescence in situ hybridization (FISH). The probes were used to investigate 
      the in situ architecture of cell aggregates of Nitrospira-like nitrite oxidizers 
      in wastewater treatment plants by FISH, confocal laser scanning microscopy, and 
      computer-aided three-dimensional visualization. Cavities and a network of 
      cell-free channels inside the Nitrospira microcolonies were detected that were 
      water permeable, as demonstrated by fluorescein staining. The uptake of different 
      carbon sources by Nitrospira-like bacteria within their natural habitat under 
      different incubation conditions was studied by combined FISH and 
      microautoradiography. Under aerobic conditions, the Nitrospira-like bacteria in 
      bioreactor samples took up inorganic carbon (as HCO(3)(-) or as CO(2)) and 
      pyruvate but not acetate, butyrate, and propionate, suggesting that these 
      bacteria can grow mixotrophically in the presence of pyruvate. In contrast, no 
      uptake by the Nitrospira-like bacteria of any of the carbon sources tested was 
      observed under anoxic or anaerobic conditions.
FAU - Daims, H
AU  - Daims H
AD  - Lehrstuhl fur Mikrobiologie, Technische Universitat Munchen, 85350 Freising, 
      Germany.
FAU - Nielsen, J L
AU  - Nielsen JL
FAU - Nielsen, P H
AU  - Nielsen PH
FAU - Schleifer, K H
AU  - Schleifer KH
FAU - Wagner, M
AU  - Wagner M
LA  - eng
SI  - GENBANK/M79383
SI  - GENBANK/X72852
SI  - GENBANK/X82559
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Nitrites)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Autoradiography
MH  - Bacteria/*classification/*genetics/growth & development
MH  - Biofilms/growth & development
MH  - Bioreactors
MH  - DNA, Bacterial/analysis/genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Nitrites/*metabolism
MH  - Oligonucleotide Probes/genetics
MH  - Oxidation-Reduction
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/*microbiology
MH  - *Waste Disposal, Fluid
PMC - PMC93301
EDAT- 2001/10/27 10:00
MHDA- 2002/01/18 10:01
PMCR- 2001/11/01
CRDT- 2001/10/27 10:00
PHST- 2001/10/27 10:00 [pubmed]
PHST- 2002/01/18 10:01 [medline]
PHST- 2001/10/27 10:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - 0850 [pii]
AID - 10.1128/AEM.67.11.5273-5284.2001 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2001 Nov;67(11):5273-84. doi: 
      10.1128/AEM.67.11.5273-5284.2001.