PMID- 11680630
OWN - NLM
STAT- MEDLINE
DCOM- 20020221
LR  - 20231213
IS  - 0031-6768 (Print)
IS  - 0031-6768 (Linking)
VI  - 442
IP  - 6
DP  - 2001 Sep
TI  - Dilated bile canaliculi and attenuated decrease of nerve-dependent bile secretion 
      in connexin32-deficient mouse liver.
PG  - 961-6
AB  - Gap junction channels in the rodent liver are composed of connexin26 (Cx26) and 
      connexin32 (Cx32) proteins. Gap junctional intercellular communication in the 
      mouse liver enhances the effects of hormonal or sympathetic stimulation of 
      glucose release from glycogen stores. To determine whether contraction of bile 
      canaliculi and bile secretion are dependent on the function of gap junction 
      channels, we compared wild-type and connexin32-deficient mice. Confocal laser 
      scanning microscopy of the wild-type mouse liver confirmed the close association 
      of connexin26 and -32 proteins with the zona occludens-1 protein and actin 
      filaments of the bile canaliculi. The decrease of bile flow after electrical 
      stimulation of sympathetic nerves in the perfused liver was attenuated in the 
      Cx32-deficient liver compared with wild-type controls. The amount of secreted 
      bile, however, was similar in wild-type and Cx32-deficient livers. Furthermore, 
      Cx32-deficient mice exhibited dilated bile canaliculi, suggesting that the 
      contraction of bile canaliculi could be impaired in these animals.
FAU - Temme, A
AU  - Temme A
AD  - Institut fur Immunologie, Medizinische Fakultat Carl Gustav Carus, TU Dresden, 
      Germany.
FAU - Stumpel, F
AU  - Stumpel F
FAU - Sohl, G
AU  - Sohl G
FAU - Rieber, E P
AU  - Rieber EP
FAU - Jungermann, K
AU  - Jungermann K
FAU - Willecke, K
AU  - Willecke K
FAU - Ott, T
AU  - Ott T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Pflugers Arch
JT  - Pflugers Archiv : European journal of physiology
JID - 0154720
RN  - 0 (Connexins)
RN  - 0 (Fluorescent Dyes)
RN  - 127120-53-0 (Connexin 26)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Aspartate Aminotransferases/blood
MH  - Bile/*metabolism/physiology
MH  - Bile Canaliculi/*pathology
MH  - Connexin 26
MH  - Connexins/analysis/*deficiency/physiology
MH  - Dilatation, Pathologic
MH  - Electric Stimulation
MH  - Fluorescent Antibody Technique
MH  - Fluorescent Dyes
MH  - Gap Junctions/chemistry/physiology
MH  - Liver/innervation/*metabolism/*pathology
MH  - Male
MH  - Mice
MH  - Microscopy, Confocal
MH  - Sympathetic Nervous System/physiopathology
MH  - Gap Junction beta-1 Protein
EDAT- 2001/10/30 10:00
MHDA- 2002/02/22 10:01
CRDT- 2001/10/30 10:00
PHST- 2001/10/30 10:00 [pubmed]
PHST- 2002/02/22 10:01 [medline]
PHST- 2001/10/30 10:00 [entrez]
AID - 10.1007/s004240100623 [doi]
PST - ppublish
SO  - Pflugers Arch. 2001 Sep;442(6):961-6. doi: 10.1007/s004240100623.