PMID- 11681578
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 930
IP  - 1-2
DP  - 2001 Sep 28
TI  - Automated dynamic sampling system for the on-line monitoring of biogenic 
      emissions from living organisms.
PG  - 39-51
AB  - An automated system for continuous on-line monitoring of biogenic emissions is 
      presented. The system is designed in such a way that volatiles, emitted as 
      reaction to biotic or abiotic stress, can be unequivocally elucidated. Two 
      identical sampling units, named target and reference bulb, are therefore 
      incorporated into the system and consecutively analyzed in monitoring 
      experiments. A number of precautions were considered during these experiments to 
      avoid the application of unwanted stress onto both organisms. Firstly, the system 
      is constructed in such a way that both bulbs are continuously flushed, i.e. 
      before, during and after analysis, with high purity air to avoid any accumulation 
      of emitted volatiles. Moreover, the air is pre-humidified by bubbling it through 
      water to sustain the biological samples for longer periods in the in vitro 
      environment. Sorptive enrichment on polydimethylsiloxane (PDMS) was used to trap 
      the headspace volatiles. The hydrophobic nature of this material permitted easy 
      removal of trapped moisture by direct flushing of the sampling cartridge with dry 
      air before desorption. The system was used to monitor the emissions from in vitro 
      mechanically wounded ivy (Hedera helix) and of in vitro grown tomato plants 
      (Lycopersicon esculentum Mill.) upon cotton leafworm (Spodoptera littoralis) 
      feeding. Differences in light and dark floral emissions of jasmine (Jasminum 
      polyanthum) were also studied.
FAU - Vercammen, J
AU  - Vercammen J
AD  - Department of Organic Chemistry, Ghent University, Belgium.
FAU - Pham-Tuan, H
AU  - Pham-Tuan H
FAU - Sandra, P
AU  - Sandra P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Biological Factors)
SB  - IM
MH  - Automation
MH  - Biological Factors/*analysis
MH  - Gas Chromatography-Mass Spectrometry/methods
MH  - Volatilization
EDAT- 2001/10/30 10:00
MHDA- 2002/03/01 10:01
CRDT- 2001/10/30 10:00
PHST- 2001/10/30 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2001/10/30 10:00 [entrez]
AID - S0021-9673(01)01172-4 [pii]
AID - 10.1016/s0021-9673(01)01172-4 [doi]
PST - ppublish
SO  - J Chromatogr A. 2001 Sep 28;930(1-2):39-51. doi: 10.1016/s0021-9673(01)01172-4.