PMID- 11681614
OWN - NLM
STAT- MEDLINE
DCOM- 20020314
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 44
IP  - 5
DP  - 2001 Oct
TI  - Molecular organization of 5S rDNA in fishes of the genus Brycon.
PG  - 893-902
AB  - There are few reports on the genomic organization of 5S rDNA in fish species. To 
      characterize the 5S rDNA nucleotide sequence and chromosomal localization in the 
      Neotropical fishes of the genus Brycon, 5S rDNA copies from seven species were 
      generated by PCR. The nucleotide sequences of the coding region (5S rRNA gene) 
      and the nontranscribed spacer (NTS) were determined, revealing that the 5S rRNA 
      genes were highly conserved, while the NTSs were widely variable among the 
      species analyzed. Moreover, two classes of NTS were detected in each species, 
      characterized by base substitutions and insertions-deletions. Using fluorescence 
      in situ hybridization (FISH), two 5S rDNA chromosome loci that could be related 
      to the two 5S rDNA NTS classes were observed in at least one of the species 
      studied. 5S rDNA sequencing and chromosomal localization permitted the 
      characterization of Brycon spp. and suggest a higher similarity among some of 
      them. The data obtained indicate that the 5S rDNA can be an useful genetic marker 
      for species identification and evolutionary studies.
FAU - Wasko, A P
AU  - Wasko AP
AD  - Departamento de Morfologia, Instituto de Biociencias, Universidade Estadual 
      Paulista, Botucatu, Sao Paulo, Brazil. awasko@ibb.unesp.br
FAU - Martins, C
AU  - Martins C
FAU - Wright, J M
AU  - Wright JM
FAU - Galetti, P M Jr
AU  - Galetti PM Jr
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brazil
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - DNA Probes
MH  - DNA, Ribosomal/*genetics
MH  - Fishes/classification/*genetics
MH  - Gene Deletion
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single Nucleotide
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Sequence Analysis, DNA
MH  - Species Specificity
EDAT- 2001/10/30 10:00
MHDA- 2002/03/15 10:01
CRDT- 2001/10/30 10:00
PHST- 2001/10/30 10:00 [pubmed]
PHST- 2002/03/15 10:01 [medline]
PHST- 2001/10/30 10:00 [entrez]
PST - ppublish
SO  - Genome. 2001 Oct;44(5):893-902.