PMID- 1168173
OWN - NLM
STAT- MEDLINE
DCOM- 19750702
LR  - 20210526
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 11
IP  - 5
DP  - 1975 May
TI  - Immunoglobulin E antibody formation in response to homologous rabbit albumin 
      heavily substituted with dinitrophenol: effect of adjuvant.
PG  - 955-61
AB  - Although much progess has been made in the detection and characterization of 
      homocytotropic antibodies, identification of the factors which control their 
      synthesis remains to be determined. To assess the influence of different 
      adjuvants on anti-dinitrophenol (DNP) immunoglobulin E (IgE) antibody responses, 
      rabbits were immunized with adjuvant plus homologous albumin (HRA) heavily 
      substituted with DNP (DNP30-HRA). This antigen in rabbits has a B cell-reactive 
      determinant (DNP) and weak non-B cell-reactive determinants (new antigenic 
      determinants) which sensitize rabbits for delayed-type hypersensitivity reactions 
      to DNP30-HRA. It was postulated that the anti-DNP IgE response to DNP30-HRA could 
      be regulated if the immunogenicity of the weak non-B cell-reactive determinants 
      (new antigenic determinants) in DNP30-HRA could be manipulated by adjuvants and 
      dosage. Complete Freund adjuvant and incomplete Freund adjuvant increased the 
      immunogenicity of the new antigenic determinants in DNP30-HRA (10 mg) much more 
      than did alum. However, equivalent primary anti-DNP IgE responses were made by 
      all rabbits sensitized with this dose, regardless of the adjuvant used. Larger 
      doses of DNP30-HRA (25 mg) in alum sensitized rabbits for strong delayed-type 
      hypersensitivity reactions to DNP30-HRA and also elicited enhanced and persistent 
      primary anti-DNP IgE responses. Enhanced but transient primary anti-DNP IgE 
      responses were elicited by 25 mg of DNP30-HRA in incomplete Freund adjuvant. In 
      contrast, no primary anti-DNP IgE responses were made to 25 mg of DNP30-HRA in 
      complete Freund adjuvant. Regardless of the adjuvant or dosage used for primary 
      immunization, no secondary anti-DNP IgE responses to DNP30-HRA were detected.
FAU - Brummer, E
AU  - Brummer E
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Dinitrophenols)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Serum Albumin)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Animals
MH  - *Antibody Formation
MH  - Dinitrophenols/*immunology
MH  - Freund's Adjuvant
MH  - Hot Temperature
MH  - Hypersensitivity, Delayed/immunology
MH  - *Immunoglobulin E
MH  - Immunoglobulin G
MH  - Immunoglobulin M
MH  - Mycobacterium/immunology
MH  - Ovalbumin
MH  - Passive Cutaneous Anaphylaxis
MH  - Rabbits/immunology
MH  - *Serum Albumin
MH  - Skin Tests
PMC - PMC415164
EDAT- 1975/05/01 00:00
MHDA- 1975/05/01 00:01
PMCR- 1975/05/01
CRDT- 1975/05/01 00:00
PHST- 1975/05/01 00:00 [pubmed]
PHST- 1975/05/01 00:01 [medline]
PHST- 1975/05/01 00:00 [entrez]
PHST- 1975/05/01 00:00 [pmc-release]
AID - 10.1128/iai.11.5.955-961.1975 [doi]
PST - ppublish
SO  - Infect Immun. 1975 May;11(5):955-61. doi: 10.1128/iai.11.5.955-961.1975.