PMID- 11682504
OWN - NLM
STAT- MEDLINE
DCOM- 20020313
LR  - 20240508
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 39
IP  - 11
DP  - 2001 Nov
TI  - Functional measurement of hepatitis C virus core-specific CD8(+) T-cell responses 
      in the livers or peripheral blood of patients by using autologous peripheral 
      blood mononuclear cells as targets or stimulators.
PG  - 3895-901
AB  - As is widely recognized, CD8(+) cytotoxic T lymphocytes (CTLs) play a crucial 
      role in hepatitis C virus (HCV) infection, both in pathogenesis of liver injury 
      and in clearing the virus. CTL studies with HCV-infected patients have been 
      difficult because of the relatively low frequency of CTL precursors in the 
      peripheral blood and because the targeted epitopes vary depending on the human 
      leukocyte antigen (HLA) types of the individuals. This study attempts to overcome 
      these problems by assessing the feasibility of using autologous peripheral blood 
      mononuclear cells (PBMCs) expressing viral antigens as stimulators or targets in 
      order to monitor the CTL responses. Primary PBMCs were transduced using a 
      retroviral vector pseudotyped with a vesicular stomatitis virus G glycoprotein 
      expressing the HCV core gene. Additionally, the vector-transduced PBMCs were used 
      as targets of CTL assays to measure the HCV core-specific CTL activities from the 
      liver-infiltrating lymphocytes of six different HLA-type patients with chronic 
      HCV infection. The core-expressing PBMCs also served as stimulators, allowing us 
      to measure core-specific CD8(+) T-cell responses by intracellular gamma 
      interferon staining of the peripheral blood of hepatitis C patients who had 
      received treatment with alpha interferon plus ribavirin. This approach provides 
      an efficient means of measuring antigen-specific CTL responses without HLA 
      constraints.
FAU - Fang, S H
AU  - Fang SH
AD  - Graduate Institute of Microbiology, College of Medicine, National Taiwan 
      University, Taipei.
FAU - Chiang, B L
AU  - Chiang BL
FAU - Wu, M H
AU  - Wu MH
FAU - Iba, H
AU  - Iba H
FAU - Lai, M Y
AU  - Lai MY
FAU - Yang, P M
AU  - Yang PM
FAU - Chen, D S
AU  - Chen DS
FAU - Hwang, L H
AU  - Hwang LH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Autoantigens)
RN  - 0 (Viral Core Proteins)
SB  - IM
MH  - Autoantigens/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology/virology
MH  - Genetic Vectors
MH  - Hepacivirus/*immunology
MH  - Hepatitis C, Chronic/*immunology/virology
MH  - Humans
MH  - Leukocytes, Mononuclear/*immunology/virology
MH  - Liver/immunology/virology
MH  - Retroviridae/genetics/metabolism
MH  - Transduction, Genetic
MH  - Vesicular stomatitis Indiana virus/genetics/metabolism
MH  - Viral Core Proteins/genetics/*immunology
PMC - PMC88461
EDAT- 2001/10/30 10:00
MHDA- 2002/03/14 10:01
PMCR- 2001/11/01
CRDT- 2001/10/30 10:00
PHST- 2001/10/30 10:00 [pubmed]
PHST- 2002/03/14 10:01 [medline]
PHST- 2001/10/30 10:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - 0508 [pii]
AID - 10.1128/JCM.39.11.3895-3901.2001 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2001 Nov;39(11):3895-901. doi: 
      10.1128/JCM.39.11.3895-3901.2001.