PMID- 11682614
OWN - NLM
STAT- MEDLINE
DCOM- 20011231
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 114
IP  - Pt 19
DP  - 2001 Oct
TI  - Noradrenaline and alpha-adrenergic signaling induce the hsp70 gene promoter in 
      mollusc immune cells.
PG  - 3557-64
AB  - Expression of heat shock proteins (hsp) is a homeostatic mechanism induced in 
      both prokaryotic and eukaryotic cells in response to metabolic and environmental 
      insults. A growing body of evidence suggests that in mammals, the hsp response is 
      integrated with physiological responses through neuroendocrine signaling. In the 
      present study, we have examined the effect of noradrenaline (NA) on the hsp70 
      response in mollusc immune cells. Oyster and abalone hemocytes transfected with a 
      gene construct containing a gastropod hsp70 gene promoter linked to the 
      luciferase reporter-gene were exposed to physiological concentrations of NA, or 
      to various alpha- and beta-adrenoceptor agonists and antagonists. Results show 
      that NA and alpha-adrenergic stimulations induced the expression of luciferase in 
      transfected mollusc immunocytes. Furthermore, exposure of hemocytes to NA or to 
      the alpha-adrenoceptor agonist phenylephrine (PE) resulted in the expression of 
      the inducible isoform of the hsp70 protein. Pertussis toxin (PTX), the 
      phospholipase C (PLC) inhibitor U73122, the protein kinase C (PKC) inhibitor 
      calphostin C, the Ca(2+)-dependent PKC inhibitor Go 6976 and the 
      phosphatidylinositol 3-kinase (PI 3-kinase) inhibitor LY294002 blocked the 
      PE-mediated induction of the hsp70 gene promoter. These results suggest that 
      alpha-adrenergic signaling induces the transcriptionnal upregulation of hsp70 in 
      mollusc hemocytes through a PTX-sensitive G-protein, PLC, Ca(2+)-dependent PKC 
      and PI 3-kinase. Thus, a functional link exists between neuroendocrine signaling 
      and the hsp70 response in mollusc immune cells.
FAU - Lacoste, A
AU  - Lacoste A
AD  - Station Biologique de Roscoff, CNRS-Universite Paris VI-INSU, Place Georges 
      Teissier, BP 74, F-29682 Roscoff Cedex, France. lacoste@sb-roscoff.fr
FAU - De Cian, M C
AU  - De Cian MC
FAU - Cueff, A
AU  - Cueff A
FAU - Poulet, S A
AU  - Poulet SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Adrenergic alpha-Agonists)
RN  - 0 (Adrenergic alpha-Antagonists)
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Adrenergic beta-Antagonists)
RN  - 0 (Carbazoles)
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Estrenes)
RN  - 0 (Flavonoids)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Indoles)
RN  - 0 (Isoquinolines)
RN  - 0 (Morpholines)
RN  - 0 (Naphthalenes)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 0 (Pyrrolidinones)
RN  - 0 (Sulfonamides)
RN  - 0 (Virulence Factors, Bordetella)
RN  - 112648-68-7 
      (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione)
RN  - 136194-77-9 (Go 6976)
RN  - 1WS297W6MV (Phenylephrine)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 9Y8NXQ24VQ (Propranolol)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.4.2.31 (Pertussis Toxin)
RN  - I271P23G24 (calphostin C)
RN  - L628TT009W (Isoproterenol)
RN  - M876330O56 (N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
RN  - X4W3ENH1CV (Norepinephrine)
RN  - XM03YJ541D (Prazosin)
SB  - IM
MH  - Adrenergic alpha-Agonists/*pharmacology
MH  - Adrenergic alpha-Antagonists/pharmacology
MH  - Adrenergic beta-Agonists/pharmacology
MH  - Adrenergic beta-Antagonists/pharmacology
MH  - Animals
MH  - Carbazoles/pharmacology
MH  - Chromones/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Estrenes/pharmacology
MH  - Flavonoids/pharmacology
MH  - Gene Expression Regulation/physiology
MH  - HSP70 Heat-Shock Proteins/*genetics
MH  - Hemocytes/cytology/*immunology
MH  - Indoles/pharmacology
MH  - Isoproterenol/pharmacology
MH  - Isoquinolines/pharmacology
MH  - Luciferases/genetics
MH  - Morpholines/pharmacology
MH  - Naphthalenes/pharmacology
MH  - Norepinephrine/*pharmacology
MH  - Ostreidae
MH  - Pertussis Toxin
MH  - Phenylephrine/pharmacology
MH  - Phosphodiesterase Inhibitors/pharmacology
MH  - Prazosin/pharmacology
MH  - Promoter Regions, Genetic/*genetics
MH  - Propranolol/pharmacology
MH  - Pyrrolidinones/pharmacology
MH  - Signal Transduction/drug effects/immunology
MH  - *Sulfonamides
MH  - Transfection
MH  - Virulence Factors, Bordetella/pharmacology
EDAT- 2001/10/30 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/30 10:00
PHST- 2001/10/30 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/30 10:00 [entrez]
AID - 10.1242/jcs.114.19.3557 [doi]
PST - ppublish
SO  - J Cell Sci. 2001 Oct;114(Pt 19):3557-64. doi: 10.1242/jcs.114.19.3557.