PMID- 11683979
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20131121
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 77
IP  - 11
DP  - 2001 Nov
TI  - A cytogenetic analysis of the long-term effect of uranium mining on peripheral 
      lymphocytes using the micronucleus-centromere assay.
PG  - 1087-93
AB  - PURPOSE: To assess the long-term effect of radiation exposure of uranium miners 
      on a cytogenetic endpoint: micronuclei (Mn) with and without a centromere. 
      MATERIALS AND METHODS: Mn were scored using the cytochalasin-B technique. It is 
      known that Mn can comprise acentric fragments or/and whole chromosomes. Mn 
      containing whole chromosomes were identified by means of fluorescence in situ 
      hybridization (FISH) with a centromere-specific probe. The frequency and 
      percentage of Mn were analysed with centromeres (MnC+) in lymphocytes of healthy 
      donors and uranium miners with large radiation exposures several decades ago 
      employed by the Wismut AG in the former German Democratic Republic. The miners 
      were subdivided into those with and those without bronchial carcinoma. RESULTS: 
      It was shown previously that the relative frequency of MnC+ decreased with dose; 
      this means that the number of Mn originating from acentric fragments increases. 
      In the study presented here, no statistically significant difference in the 
      overall Mn frequency was seen between the analysed groups. The fraction of MnC+, 
      however, was highest in lymphocytes of healthy male donors (mean: 74.6%) followed 
      by healthy miners (mean: 62.1%) and those suffering from cancer (mean: 55.8%). 
      CONCLUSION: The results indicate the occurrence of a genomic instability in 
      lymphocytes of miners, especially those with cancer. It appears that the low 
      percentage of MnC+ may be a marker of genomic instability and cancer 
      predisposition.
FAU - Kryscio, A
AU  - Kryscio A
AD  - Institut fur Medizinische Strahlenbiologie, Universitatsklinikum Essen, D-45122 
      Essen, Germany.
FAU - Ulrich Muller, W U
AU  - Ulrich Muller WU
FAU - Wojcik, A
AU  - Wojcik A
FAU - Kotschy, N
AU  - Kotschy N
FAU - Grobelny, S
AU  - Grobelny S
FAU - Streffer, C
AU  - Streffer C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 4OC371KSTK (Uranium)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Centromere/genetics/radiation effects
MH  - Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - Lung Neoplasms/genetics
MH  - Lymphocytes/drug effects/*radiation effects
MH  - Male
MH  - *Micronucleus Tests
MH  - Middle Aged
MH  - Mining
MH  - *Occupational Exposure
MH  - Uranium/*toxicity
EDAT- 2001/10/31 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/31 10:00
PHST- 2001/10/31 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/31 10:00 [entrez]
AID - 10.1080/09553000110070289 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2001 Nov;77(11):1087-93. doi: 10.1080/09553000110070289.