PMID- 11683983
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20061115
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 77
IP  - 11
DP  - 2001 Nov
TI  - Analysis of chromosome aberrations by FISH and Giemsa assays in lymphocytes of 
      cancer patients undergoing whole-body irradiation: comparison of in vivo and in 
      vitro irradiation.
PG  - 1123-31
AB  - PURPOSE: To study the cytogenetic effects of fractionated radiotherapy in 
      peripheral blood lymphocytes of five cancer patients. In vitro experiments were 
      performed in parallel using the same dose range and a comparison was made of the 
      induced frequencies of stable and unstable chromosome aberrations. The object was 
      to clarify the use of an in vitro calibration curve for immediate and 
      retrospective dosimetry in cases of radiation accidents. MATERIALS AND METHODS: 
      Patients were exposed to 60Co gamma-rays at a single dose of 11.5 cGy each day up 
      to a total dose of 57.5 cGy, given in 5 days. For measurement of chromosome 
      aberrations, blood was collected from patients before irradiation and after each 
      exposure. Blood taken before treatment was used as a control and for in vitro 
      irradiation experiments in the dose range 8-50 cGy. Chromosome aberration 
      frequency (stable as well as unstable) was determined using fluorescence in situ 
      hybridization (FISH) assay with specific DNA libraries for chromosomes 1, 4 and 8 
      and a pancentromertic probe for the whole genome. Giemsa-stained preparations 
      were used to score unstable aberrations following in vivo and in vitro exposure. 
      RESULTS: A linear dose-response curve was determined for both dicentrics and 
      translocations. The in vivo frequency of translocations was higher than for 
      dicentrics. Dose-response curves generated for translocations following in vivo 
      and in vitro irradiation yielded similar frequencies. In contrast, for 
      dicentrics, in vitro irradiation yielded a higher frequency when compared with 
      data generated following in vivo exposure. CONCLUSIONS: For dose reconstruction 
      purposes, translocations frequency seems to be a more adequate end-point than the 
      scoring of dicentrics. The established in vitro calibration curve for dicentrics 
      may underestimate absorbed radiation dose in cases of protracted exposure.
FAU - Vorobtsova, I
AU  - Vorobtsova I
AD  - Laboratory of Radiation Genetics, Central Research Institute of Roentgenology and 
      Radiology, Pesochny, Leningradskaya 70/4, 197758, St Petersburg, Russia. 
      radgen@gate.la.spb.ru
FAU - Darroudi, F
AU  - Darroudi F
FAU - Semyonov, A
AU  - Semyonov A
FAU - Kanayeva, A
AU  - Kanayeva A
FAU - Timofeyeva, N
AU  - Timofeyeva N
FAU - Yakovleva, T
AU  - Yakovleva T
FAU - Zharinov, G
AU  - Zharinov G
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (Azure Stains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Azure Stains
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis/methods
MH  - Dose-Response Relationship, Radiation
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*physiology/*radiation effects
MH  - Male
MH  - Middle Aged
MH  - Neoplasms/*genetics/*radiotherapy
MH  - *Whole-Body Irradiation
EDAT- 2001/10/31 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/31 10:00
PHST- 2001/10/31 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/31 10:00 [entrez]
AID - 10.1080/09553000110075527 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 2001 Nov;77(11):1123-31. doi: 10.1080/09553000110075527.