PMID- 11684118
OWN - NLM
STAT- MEDLINE
DCOM- 20011214
LR  - 20191210
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 258
IP  - 1-2
DP  - 2001 Dec 1
TI  - International Standards for hepatocyte growth factor/scatter factor: initial 
      assessment of candidate materials and their evaluation by multicentre 
      collaborative study.
PG  - 1-11
AB  - Hepatocyte growth factor/scatter factor (HGF/SF) is a potent paracrine growth 
      factor with motogenic, mitogenic and morphogenic activities, and a potential 
      therapeutic role in hepatic and renal disease, as well as diagnostic and 
      prognostic applications. It is synthesised as an inactive, single-chain precursor 
      that is cleaved by serine proteases to give a biologically active, heterodimeric 
      form. To develop World Health Organization (WHO) International Standards (IS) for 
      HGF/SF, candidate preparations of the two forms were assessed in a multicentre 
      study in which they were compared with local standards by bioassay and 
      immunoassay. Among laboratories, there was a wide variation in the estimates of 
      potencies of the candidate standards in terms of in-house reference preparations, 
      but between-assay and within-assay variabilities were low within laboratories. In 
      some assay systems, the precursor and heterodimer showed different responses. 
      Since both molecular forms are widely used in current assay systems, this 
      suggested that a reference preparation was required for each form of the HGF/SF 
      molecule. Accordingly, the Expert Committee on Biological Standardization of WHO 
      established the heterodimeric material (96/564) as the first IS for HGF/SF, 
      human, recombinant, with an assigned unitage of 4000 IU/ampoule and, for the 
      purpose of immunoassay calibration, a nominal HGF/SF content of 4 microg/ampoule. 
      The precursor preparation (96/556) was established as the first IS for HGF/SF 
      (precursor) with an assigned unitage of 2000 IU/ampoule and, for the purpose of 
      immunoassay calibration, a nominal HGF/SF (precursor) content of 4 
      microg/ampoule. The preparations can be obtained upon written request to the 
      National Institute for Biological Standards and Control (NIBSC, PO Box 1193), by 
      e-mail (standards@nibsc.ac.uk) or ordered at http://www.nibsc.ac.uk.
FAU - Rafferty, B
AU  - Rafferty B
AD  - Division of Endocrinology, National Institute for Biological Standards and 
      Control, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3QG, UK. 
      brafferty@nibsc.ac.uk
FAU - Maile, P
AU  - Maile P
FAU - Rigsby, P
AU  - Rigsby P
FAU - Gaines Das, R E
AU  - Gaines Das RE
FAU - Robinson, C J
AU  - Robinson CJ
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Multicenter Study
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Protein Precursors)
RN  - 0 (Recombinant Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Biological Assay/standards
MH  - CHO Cells
MH  - Cell Line
MH  - Cricetinae
MH  - Dimerization
MH  - Hepatocyte Growth Factor/chemistry/*standards
MH  - Humans
MH  - Immunoassay/standards
MH  - International Cooperation
MH  - Mice
MH  - Protein Precursors/chemistry/standards
MH  - Protein Structure, Quaternary
MH  - Recombinant Proteins/chemistry/standards
MH  - Reference Standards
MH  - World Health Organization
EDAT- 2001/10/31 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/31 10:00
PHST- 2001/10/31 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/31 10:00 [entrez]
AID - S0022-1759(01)00482-3 [pii]
AID - 10.1016/s0022-1759(01)00482-3 [doi]
PST - ppublish
SO  - J Immunol Methods. 2001 Dec 1;258(1-2):1-11. doi: 10.1016/s0022-1759(01)00482-3.