PMID- 11685476
OWN - NLM
STAT- MEDLINE
DCOM- 20011226
LR  - 20071115
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 53
IP  - 7
DP  - 2001 Sep
TI  - High-resolution mapping of the human 4q21 and the mouse 5E3 SCYB chemokine 
      cluster by fiber-fluorescence in situ hybridization.
PG  - 611-5
AB  - The CXC chemokine or small inducible cytokine B (SCYB) subfamily includes the 
      T-cell chemoattractants MIG (CXCL9, SCYB9), IP-10 (CXCL10, SCYB10), and I-TAC 
      (CXCL11, SCYB11). These three highly homologous chemokines lack the glutamic 
      acid-leucine-arginine (ELR) motif and signal via the CXCR3 receptor. Previous 
      work showed that the genes encoding these chemokines are localized in an 
      individual mini-cluster on human Chromosome (Chr) 4 at position 4q21.2. Recently, 
      we identified mouse Scyb11 and mapped this gene by fluorescence in situ 
      hybridization (FISH) to mouse Chr 5E3, the orthologous locus to human 4q21 where 
      the other two homologous mouse genes, Scyb9 and Scyb10, have also been localized. 
      Since SCYB10 and SCYB11 are not represented in the recently published draft 
      sequence of the human genome, we wanted to clarify exactly the order and 
      distances of the three chemokine genes using two-color FISH on stretched DNA 
      fiber preparations. Here, we report the simultaneous localization of all three 
      genes and provide high-resolution visual maps of this chemokine cluster from both 
      mouse and human. The three chemokine genes were found within a range of 32 kb on 
      mouse and 29 kb on human DNA fiber targets. The precise physical distances were 
      defined, and an almost identical arrangement of the human and mouse homologues 
      was identified, indicating that this CXC chemokine mini-cluster has been 
      completely conserved evolutionarily since the divergence of mouse and human. Our 
      results refine previous maps of the three genes, support the hypothesis that they 
      resulted from gene duplication that took place in a common ancestor of mouse and 
      human, and provide complementary information on a region of the draft sequence of 
      human Chr 4 that is not yet covered.
FAU - Erdel, M
AU  - Erdel M
AD  - Institute of Medical Biology and Human Genetics, University of Innsbruck, 
      Schopfstrasse 41, 6020 Innsbruck, Austria. martin.erdel@uibk.ac.at
FAU - Theurl, M
AU  - Theurl M
FAU - Meyer, M
AU  - Meyer M
FAU - Duba, H C
AU  - Duba HC
FAU - Utermann, G
AU  - Utermann G
FAU - Werner-Felmayer, G
AU  - Werner-Felmayer G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (CXCL10 protein, human)
RN  - 0 (CXCL11 protein, human)
RN  - 0 (CXCL9 protein, human)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Chemokine CXCL11)
RN  - 0 (Chemokine CXCL9)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl10 protein, mouse)
RN  - 0 (Cxcl11 protein, mouse)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
SB  - IM
MH  - Animals
MH  - Chemokine CXCL10
MH  - Chemokine CXCL11
MH  - Chemokine CXCL9
MH  - Chemokines, CXC/*genetics
MH  - Chromosomes, Human, Pair 4/*genetics
MH  - Evolution, Molecular
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Mice/*genetics
MH  - *Multigene Family
MH  - Species Specificity
EDAT- 2001/10/31 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/10/31 10:00
PHST- 2001/05/08 00:00 [received]
PHST- 2001/10/31 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/10/31 10:00 [entrez]
AID - 10.1007/s002510100363 [doi]
PST - ppublish
SO  - Immunogenetics. 2001 Sep;53(7):611-5. doi: 10.1007/s002510100363.