PMID- 11685533
OWN - NLM
STAT- MEDLINE
DCOM- 20020502
LR  - 20220331
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 110
IP  - 5
DP  - 2001 Sep
TI  - The centromere structure in Robertsonian wheat-rye translocation chromosomes 
      indicates that centric breakage-fusion can occur at different positions within 
      the primary constriction.
PG  - 335-44
AB  - Univalent chromosomes at meiotic metaphase I have a tendency to misdivide at the 
      centromeres. Fusion of the misdivision products may produce Robertsonian 
      translocations. The fine structure of the centromeres in Robertsonian wheat-rye 
      translocation chromosomes was analyzed by fluorescence in situ hybridization 
      (FISH) using two centromere-specific DNA clones: pRCS1, derived from rice, and 
      pAWRC1, derived from rye. Clone pRCS1 hybridizes to the centromeres of all 
      grasses including wheat and rye, whereas clone pAWRC1 is rye specific and 
      hybridizes only to the centromeres of rye. Four of the six wheat-rye 
      translocations derived from a single centric misdivision event (1st generation 
      translocations) had hybrid centromeres, with approximately half of the centromere 
      derived from rye and half from wheat. In the two other 1st generation 
      translocations, the entire centromere was derived from rye. Among eight 
      reconstructed wheat and rye chromosomes that originated from two consecutive 
      centric misdivision-fusion events (2nd generation translocations), T1BS.1BL 
      (derived from T1BS.1RL and T1RS.1BL) and one of three T2BS.2BL (derived from 
      T2RS.2BL and T2BS.2RL) had hybrid centromeres. T1RS.1RL (derived from T1BS.1RL 
      and T1RS.1BL), two of three T2BS.2BL, and all three T2RS.2RL (derived from 
      T2RS.2BL and T2BS.2RL) had rye centromeres. All three 3rd generation 
      translocations had hybrid centromeres with approximately half of the centromere 
      derived from rye. There were no indications that the composite structure of the 
      centromere in these chromosomes affected their behavior in mitosis or meiosis. 
      These observations support the notion of a compound structure of the centromere 
      in higher organisms, and indicate that during the centric breakage-fusion event, 
      centromere breakage may occur in different positions along the segment of the 
      chromosome that interacts with the spindle fibers. Normal behavior of the 1st, 
      2nd, and 3rd generation centric translocations in mitosis and meiosis indicates 
      that, at least in wheat and rye, centromeres are not chromosome specific.
FAU - Zhang, P
AU  - Zhang P
AD  - Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State 
      University, Manhattan 66506-5502, USA.
FAU - Friebe, B
AU  - Friebe B
FAU - Lukaszewski, A J
AU  - Lukaszewski AJ
FAU - Gill, B S
AU  - Gill BS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - Cell Line
MH  - *Centromere/ultrastructure
MH  - *Chromosome Breakage
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Secale/*genetics
MH  - *Translocation, Genetic
MH  - Triticum/*genetics
EDAT- 2001/10/31 10:00
MHDA- 2002/05/03 10:01
CRDT- 2001/10/31 10:00
PHST- 2001/01/16 00:00 [received]
PHST- 2001/06/08 00:00 [accepted]
PHST- 2001/10/31 10:00 [pubmed]
PHST- 2002/05/03 10:01 [medline]
PHST- 2001/10/31 10:00 [entrez]
AID - 10.1007/s004120100159 [doi]
PST - ppublish
SO  - Chromosoma. 2001 Sep;110(5):335-44. doi: 10.1007/s004120100159.