PMID- 11685658
OWN - NLM
STAT- MEDLINE
DCOM- 20020115
LR  - 20191105
IS  - 0918-4287 (Print)
IS  - 0918-4287 (Linking)
VI  - 34
IP  - 2
DP  - 2001 Jun
TI  - The protective effect of hepatocyte growth-promoting factor (pHGF) against 
      hydrogen peroxide-induced acute lung injury in rats.
PG  - 92-102
AB  - To examine the protective effect of hepatocyte growth-promoting factor (pHGF) in 
      hydrogen peroxide (H(2)O(2))-induced acute lung injury in rats, we observed the 
      pathological changes in lung tissue by terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) and by light and electron 
      microscopy. We also measured the serum levels of lipid peroxide (LPO). At 6 to 24 
      h after H(2)O(2) injection, the level of LPO was significantly higher in the 
      H(2)O(2) group than in the H(2)O(2) + pHGF-treated group. This finding indicated 
      that pHGF protected against cell membrane damage in H2O2-induced acute lung 
      injury. Positive TUNEL signals were found in capillary endothelial cells, 
      alveolar epithelial cells, and inflammatory cells. In the H(2)O(2) + pHGF-treated 
      group, TUNEL-positive signals were reduced compared with those in the H(2)O(2) 
      group. This finding indicated that pHGF acts to suppress apoptosis. In the 
      H(2)O(2) group, severe pulmonary edema was seen 3 h after H(2)O(2) injection, and 
      at 24 h, severe atelectasis was seen. In the H(2)O(2) + pHGF-treated group, 
      pulmonary edema was scarcely seen and severe atelectasis was not found. This 
      finding indicated that pHGF acts to suppress both severe pulmonary edema and 
      atelectasis. In the H(2)O(2) group, the formation of subendothelial blebs and 
      disruption of endothelial cells was observed. Edema and disruption were seen in 
      type I epithelial cells. In type II lung epithelial cells, mitochondria were 
      swollen and microvilli had disappeared. In the H(2)O(2) + pHGF-treated group, the 
      formation of subendothelial blebs was seen, but no severe subendothelial blebs 
      were observed. Disruption of capillary endothelial cells and type I epithelial 
      cells was not evident, nor was there damage to type II lung epithelial cells. 
      These findings indicated that pHGF protects the progression of H(2)O(2)-induced 
      acute lung injury, and showed that pHGF acts to stabilize the cell membrane in 
      capillary endothelial cells and lung epithelial cells.
FAU - Liu, X L
AU  - Liu XL
AD  - First Department of Pathology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, 
      Tokyo 113-8603, Japan. xiao-lan@nms.ac.jp
FAU - Sato, S
AU  - Sato S
FAU - Dai, W
AU  - Dai W
FAU - Yamanaka, N
AU  - Yamanaka N
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Med Electron Microsc
JT  - Medical electron microscopy : official journal of the Clinical Electron 
      Microscopy Society of Japan
JID - 9430789
RN  - 0 (Lipid Peroxides)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Membrane/drug effects
MH  - Endothelium, Vascular/drug effects/ultrastructure
MH  - Epithelium/drug effects/ultrastructure
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Hydrogen Peroxide
MH  - In Situ Nick-End Labeling
MH  - Lipid Peroxides/blood
MH  - Lung Diseases/blood/chemically induced/*prevention & control
MH  - Microscopy, Electron
MH  - Pulmonary Alveoli/drug effects/pathology/ultrastructure
MH  - Pulmonary Edema/chemically induced
MH  - Rats
MH  - Time Factors
EDAT- 2001/10/31 10:00
MHDA- 2002/01/16 10:01
CRDT- 2001/10/31 10:00
PHST- 2001/05/01 00:00 [received]
PHST- 2001/06/18 00:00 [accepted]
PHST- 2001/10/31 10:00 [pubmed]
PHST- 2002/01/16 10:01 [medline]
PHST- 2001/10/31 10:00 [entrez]
AID - 10.1007/s007950170003 [doi]
PST - ppublish
SO  - Med Electron Microsc. 2001 Jun;34(2):92-102. doi: 10.1007/s007950170003.