PMID- 11688548
OWN - NLM
STAT- MEDLINE
DCOM- 20011204
LR  - 20131121
IS  - 0323-9950 (Print)
IS  - 0323-9950 (Linking)
VI  - 25
IP  - 3-4
DP  - 2000
TI  - Changes of renal function and blood pressure after nitric oxide synthase 
      inhibition in renal-denervated conscious rats.
PG  - 109-14
AB  - Conscious male Wistar rats with implanted catheters in femoral artery for blood 
      pressure registration, femoral vein for drug infusion and bladder for urine 
      collection were used. Nitric oxide synthase inhibition (NOSI) was performed by 
      intravenous administration of 10 mg/kg b.w. N(omega)-nitro-L-arginine methyl 
      ester (L-NAME) before and one week after bilateral renal denervation. Renal 
      denervation led to a decrease of urine osmolality (p<0.05). NOSI decreased heart 
      rate (p<0.001) and increased systolic, mean and diastolic artery pressure both in 
      the intact and the renal-denervated rats (p<0.001). The P(MF)/P(HF) ratio in the 
      heart rate spectrum, considered a criterion for the sympathovagal balance 
      decreased after NOSI in intact and in renal-denervated rats (p<0.001) indicating 
      a reduction in the sympathetic tone. The baroreflex sensitivity after NOSI 
      increased both in conscious rats with intact renal nerves and in rats with 
      bilateral renal denervation. In intact rats NOSI increased urine flow rate by 
      48.7% (p<0.05), sodium excretion by 339.7% (p<0.01) and chloride excretion by 
      272.1% (p<0.01). In contrast to the intact rats, NOSI in the renal denervated 
      rats decreased their urine flow rate by 35.5% (p<0.05) and did not alter sodium 
      and chloride excretion. So, chronic renal denervation inversed the diuretic and 
      clearly attenuated the natriuretic and chloruretic response to acute NOSI without 
      inhibiting the rise in blood pressure. Therefore, the increased urine flow as 
      well as the sodium and chloride excretion observed after NOSI are not exclusively 
      the result of a pressure diuresis but are somewhat dependent on the renal 
      sympathetic nerve activity. Renal denervation did not change the pattern of 
      sympathovagal balance and baroreflex sensitivity modified by NO-synthase 
      inhibition.
FAU - Girchev, R
AU  - Girchev R
AD  - Department of Physiology, Medical University, Sofia, Bulgaria. 
      girchev@medfac.acad.bg
FAU - Mikhov, D
AU  - Mikhov D
FAU - Markova, P
AU  - Markova P
FAU - Vuchidolova, V
AU  - Vuchidolova V
LA  - eng
PT  - Journal Article
PL  - Bulgaria
TA  - Acta Physiol Pharmacol Bulg
JT  - Acta physiologica et pharmacologica Bulgarica
JID - 7512568
RN  - 0 (Enzyme Inhibitors)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Animals
MH  - Baroreflex/drug effects
MH  - Blood Pressure/drug effects
MH  - Denervation
MH  - Enzyme Inhibitors/*pharmacology
MH  - Heart Rate/drug effects
MH  - Kidney/drug effects/innervation/*physiology
MH  - Male
MH  - Nitric Oxide/physiology
MH  - Nitric Oxide Synthase/*antagonists & inhibitors
MH  - Rats
MH  - Rats, Wistar
MH  - Urodynamics/drug effects
EDAT- 2001/11/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/02 10:00
PHST- 2001/11/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/02 10:00 [entrez]
PST - ppublish
SO  - Acta Physiol Pharmacol Bulg. 2000;25(3-4):109-14.