PMID- 11688991
OWN - NLM
STAT- MEDLINE
DCOM- 20011218
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 288
IP  - 4
DP  - 2001 Nov 9
TI  - Identification of genes differentially induced by hypoxia in pancreatic cancer 
      cells.
PG  - 882-6
AB  - A hypoxic microenvironment is characteristic of many solid tumors, including 
      pancreatic cancer, the fifth leading cause of cancer death in the United States. 
      Hypoxia causes the stabilization of the HIF-1 (hypoxia-inducible factor-1) 
      transcription factor and the induction of many genes that promote angiogenesis, 
      tumor growth, and metastasis. We performed representational difference analysis 
      (RDA) using mRNA extracted from hypoxic and normoxic Capan-2, a human pancreatic 
      cancer cell line. cDNAs corresponding to hypoxia-inducible genes were cloned and 
      sequenced. We identified GPI/NLK/AMF (glucose phosphate 
      isomerase/neuroleukin/autocrine motility factor) as a hypoxic inducible gene. In 
      addition, hexokinase II and DEC1/Stra13, genes known to be hypoxia inducible in 
      other systems, were found to be hypoxia inducible in our pancreatic cancer 
      system. We thus identified three genes that are induced by hypoxia in a human 
      pancreatic cancer, including GPI/NLK/AMF, which was not previously known to be 
      hypoxia inducible in any other system. These genes may provide new targets for 
      diagnosis and treatment of pancreatic cancer.
CI  - Copyright 2001 Academic Press.
FAU - Yoon, D Y
AU  - Yoon DY
AD  - Department of Pathology, University of California, Los Angeles 90095, USA.
FAU - Buchler, P
AU  - Buchler P
FAU - Saarikoski, S T
AU  - Saarikoski ST
FAU - Hines, O J
AU  - Hines OJ
FAU - Reber, H A
AU  - Reber HA
FAU - Hankinson, O
AU  - Hankinson O
LA  - eng
GR  - R01CA28868/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (BHLHE40 protein, human)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Basic Helix-Loop-Helix Transcription Factors
MH  - Cell Hypoxia/drug effects/*genetics
MH  - DNA-Binding Proteins/metabolism
MH  - *Gene Expression Regulation, Neoplastic/drug effects
MH  - Glucose-6-Phosphate Isomerase/genetics
MH  - Hexokinase/genetics
MH  - Homeodomain Proteins/genetics
MH  - Humans
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Nuclear Proteins/metabolism
MH  - Oxygen/metabolism/pharmacology
MH  - Pancreatic Neoplasms/enzymology/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - *Transcription Factors
MH  - Tumor Cells, Cultured
MH  - *Up-Regulation/drug effects
EDAT- 2001/11/02 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/02 10:00
PHST- 2001/11/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/02 10:00 [entrez]
AID - S0006-291X(01)95867-X [pii]
AID - 10.1006/bbrc.2001.5867 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Nov 9;288(4):882-6. doi: 10.1006/bbrc.2001.5867.