PMID- 11689429
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20220309
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 20
IP  - 21
DP  - 2001 Nov 1
TI  - Synaptic secretion of BDNF after high-frequency stimulation of glutamatergic 
      synapses.
PG  - 5887-97
AB  - The protein brain-derived neurotrophic factor (BDNF) has been postulated to be a 
      retrograde or paracrine synaptic messenger in long-term potentiation and other 
      forms of activity-dependent synaptic plasticity. Although crucial for this 
      concept, direct evidence for the activity-dependent synaptic release of BDNF is 
      lacking. Here we investigate secretion of BDNF labelled with green fluorescent 
      protein (BDNF-GFP) by monitoring the changes in fluorescence intensity of 
      dendritic BDNF-GFP vesicles at glutamatergic synaptic junctions of living 
      hippocampal neurons. We show that high-frequency activation of glutamatergic 
      synapses triggers the release of BDNF-GFP from synaptically localized secretory 
      granules. This release depends on activation of postsynaptic ionotropic glutamate 
      receptors and on postsynaptic Ca(2+) influx. Release of BDNF-GFP is also observed 
      from extrasynaptic dendritic vesicle clusters, suggesting that a possible spatial 
      restriction of BDNF release to specific synaptic sites can only occur if the 
      postsynaptic depolarization remains local. These results support the concept of 
      BDNF being a synaptic messenger of activity-dependent synaptic plasticity, which 
      is released from postsynaptic neurons.
FAU - Hartmann, M
AU  - Hartmann M
AD  - Department of Molecular Neurobiochemistry, Ruhr University Bochum, NC7-132, 
      D-44780 Bochum, Germany.
FAU - Heumann, R
AU  - Heumann R
FAU - Lessmann, V
AU  - Lessmann V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/genetics/*metabolism
MH  - Cells, Cultured
MH  - Dendrites/metabolism/ultrastructure
MH  - Electric Stimulation/methods
MH  - Glutamic Acid/*metabolism
MH  - Green Fluorescent Proteins
MH  - Hippocampus/cytology/metabolism
MH  - Luminescent Proteins/genetics
MH  - Membrane Potentials/drug effects
MH  - Microscopy, Video
MH  - Neurons/cytology/metabolism
MH  - Potassium/metabolism/pharmacology
MH  - Rats
MH  - Recombinant Fusion Proteins/biosynthesis/genetics/metabolism
MH  - Secretory Vesicles/metabolism
MH  - Synapses/*metabolism
MH  - Synaptic Vesicles/metabolism
MH  - Transfection
PMC - PMC125691
EDAT- 2001/11/02 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/11/01
CRDT- 2001/11/02 10:00
PHST- 2001/11/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/02 10:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - cde578 [pii]
AID - 10.1093/emboj/20.21.5887 [doi]
PST - ppublish
SO  - EMBO J. 2001 Nov 1;20(21):5887-97. doi: 10.1093/emboj/20.21.5887.