PMID- 11689646
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20240315
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 75
IP  - 23
DP  - 2001 Dec
TI  - Gene expression profile of herpesvirus-infected T cells obtained using 
      immunomicroarrays: induction of proinflammatory mechanisms.
PG  - 11641-50
AB  - Herpesvirus infections can frequently lead to acute inflammation, yet the 
      mechanisms regulating this event remain poorly understood. In order to determine 
      some of the immunological mechanisms regulated by human herpesvirus infections, 
      we studied the gene expression profile of lymphocytes infected with human 
      herpesvirus 6 (HHV-6) by using a novel immunomicroarray. Our nylon-based 
      immunomicroarray contained more than 1,150 immune response-related genes and was 
      highly consistent between experiments. Experimentally, we found that 
      independently of the HHV-6 strain used to infect T cells, multiple 
      proinflammatory genes were increased and anti-inflammatory genes were decreased 
      at the mRNA and protein levels. HHV-6 strains A and B increased expression of the 
      genes for interleukin-18 (IL-18), the IL-2 receptor, members of the tumor 
      necrosis factor alpha superfamily receptors, mitogen-activated protein kinase, 
      and Janus kinase signaling proteins. As reported previously, CD4 protein levels 
      were also increased significantly. Specific type 2 cytokines, including IL-10, 
      its receptor, and IL-14, were downregulated by HHV-6 infection and, 
      interestingly, amyloid precursor proteins and type 1 and 2 presenilins. Thus, T 
      cells respond to HHV-6 infection by inducing a type 1 immune response that may 
      play a significant role in the development and progression of diseases associated 
      with HHV-6, including pediatric, hematologic, transplant, and neurologic 
      disorders.
FAU - Mayne, M
AU  - Mayne M
AD  - Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, 
      Manitoba, Canada. mmayne@cc.umanitoba.ca
FAU - Cheadle, C
AU  - Cheadle C
FAU - Soldan, S S
AU  - Soldan SS
FAU - Cermelli, C
AU  - Cermelli C
FAU - Yamano, Y
AU  - Yamano Y
FAU - Akhyani, N
AU  - Akhyani N
FAU - Nagel, J E
AU  - Nagel JE
FAU - Taub, D D
AU  - Taub DD
FAU - Becker, K G
AU  - Becker KG
FAU - Jacobson, S
AU  - Jacobson S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (CD4 Antigens)
RN  - 0 (DNA Primers)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Base Sequence
MH  - CD4 Antigens/genetics
MH  - Cell Line
MH  - DNA Primers
MH  - Down-Regulation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Gene Expression Profiling
MH  - *Genes, Viral
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Interleukin-18/genetics
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polymerase Chain Reaction
MH  - T-Lymphocytes/*metabolism/virology
MH  - Up-Regulation
PMC - PMC114751
EDAT- 2001/11/02 10:00
MHDA- 2002/01/05 10:01
PMCR- 2001/12/01
CRDT- 2001/11/02 10:00
PHST- 2001/11/02 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/02 10:00 [entrez]
PHST- 2001/12/01 00:00 [pmc-release]
AID - 1022 [pii]
AID - 10.1128/JVI.75.23.11641-11650.2001 [doi]
PST - ppublish
SO  - J Virol. 2001 Dec;75(23):11641-50. doi: 10.1128/JVI.75.23.11641-11650.2001.