PMID- 11696575
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 108
IP  - 9
DP  - 2001 Nov
TI  - The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte 
      arrest on early atherosclerotic endothelium.
PG  - 1307-14
AB  - In a reconstituted flow chamber system, preincubation with chemokines can trigger 
      the arrest of rolling monocytes, suggesting that this interaction could help 
      recruit these cells to early atherosclerotic lesions. To date, however, the 
      contribution of endothelium-derived chemokines found in these lesion to monocyte 
      arrests has not been investigated. The endothelium of lesion-prone carotid 
      arteries from apolipoprotein E-deficient (ApoE(-/-)) mice, but not control mice, 
      presents the chemokines KC (mouse GRO-alpha) and JE (mouse monocyte 
      chemoattractant protein-1 [MCP-1]). Arrest of a monocytic cell line or mouse 
      blood monocytes perfused through carotid arteries of ApoE(-/-) mice was reduced 
      by treating with either pertussis toxin, an antagonist of CXCR2, or an antibody 
      to KC, but this process was insensitive to agents that blocked CCR-2 or JE. 
      Conversely, monocyte accumulation more than doubled upon pre-perfusion of the 
      carotid artery with KC but not with mouse MCP-1. Blockade of alpha(4)beta(1) 
      integrin (VLA-4) or vascular cell adhesion molecule-1, but not CD18 or 
      intercellular adhesion molecule-1, almost completely inhibited the arrest of 
      monocytes. We conclude that when presented by early atherosclerotic lesions, KC 
      but not murine MCP-1 triggers VLA-4-dependent monocyte recruitment.
FAU - Huo, Y
AU  - Huo Y
AD  - Cardiovascular Research Center and Department of Biomedical Engineering, 
      University of Virginia, Health Science Center, Charlottesville, Virginia 22908, 
      USA.
FAU - Weber, C
AU  - Weber C
FAU - Forlow, S B
AU  - Forlow SB
FAU - Sperandio, M
AU  - Sperandio M
FAU - Thatte, J
AU  - Thatte J
FAU - Mack, M
AU  - Mack M
FAU - Jung, S
AU  - Jung S
FAU - Littman, D R
AU  - Littman DR
FAU - Ley, K
AU  - Ley K
LA  - eng
GR  - R01 HL058108/HL/NHLBI NIH HHS/United States
GR  - HL-58108/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD18 Antigens)
RN  - 0 (CXCL1 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL1)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cxcl1 protein, mouse)
RN  - 0 (Cxcl1 protein, rat)
RN  - 0 (Growth Substances)
RN  - 0 (Integrin alpha4beta1)
RN  - 0 (Integrins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Receptors, Interleukin-8B)
RN  - 0 (Receptors, Lymphocyte Homing)
RN  - 0 (Virulence Factors, Bordetella)
RN  - EC 2.4.2.31 (Pertussis Toxin)
SB  - IM
CIN - J Clin Invest. 2001 Nov;108(9):1269-71. PMID: 11696568
MH  - Animals
MH  - Antibodies, Monoclonal/metabolism
MH  - Arteriosclerosis/*metabolism
MH  - CD18 Antigens/metabolism
MH  - Carotid Arteries/metabolism
MH  - Cell Adhesion
MH  - Cell Line
MH  - Chemokine CCL2/*metabolism/*physiology
MH  - Chemokine CXCL1
MH  - *Chemokines, CXC
MH  - Chemotactic Factors/*metabolism/*physiology
MH  - Endothelium, Vascular/*metabolism
MH  - Growth Substances/*metabolism/*physiology
MH  - Humans
MH  - Integrin alpha4beta1
MH  - Integrins/metabolism
MH  - *Intercellular Signaling Peptides and Proteins
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/*metabolism/*physiology
MH  - Pertussis Toxin
MH  - Protein Binding
MH  - Rats
MH  - Receptors, Interleukin-8B/antagonists & inhibitors
MH  - Receptors, Lymphocyte Homing/metabolism
MH  - Time Factors
MH  - Virulence Factors, Bordetella/pharmacology
PMC - PMC209441
EDAT- 2001/11/07 10:00
MHDA- 2002/01/05 10:01
PMCR- 2001/11/01
CRDT- 2001/11/07 10:00
PHST- 2001/11/07 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/07 10:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - 12877 [pii]
AID - 10.1172/JCI12877 [doi]
PST - ppublish
SO  - J Clin Invest. 2001 Nov;108(9):1307-14. doi: 10.1172/JCI12877.