PMID- 11696873
OWN - NLM
STAT- MEDLINE
DCOM- 20011219
LR  - 20191025
IS  - 0895-8378 (Print)
IS  - 0895-8378 (Linking)
VI  - 13
IP  - 11
DP  - 2001 Nov
TI  - Effects of the inhalation of diesel exhaust, Kanto loam dust, or diesel exhaust 
      without particles on immune responses in mice exposed to Japanese cedar 
      (Cryptomeria japonica) pollen.
PG  - 1047-63
AB  - To assess the potential enhancement by air-pollutants of immune responses in 
      mice, especially with regard to allergen-specific immunoglobulin E (IgE) antibody 
      production, female BDF(1) mice (60 mice in each group) were exposed to diesel 
      exhaust (particles, 3.24 mg/m(3); nitrogen dioxide, 1.0 ppm: DE group), Kanto 
      loam dust (particles, 3.29 mg/m(3); nitrogen dioxide, 0.01 ppm: KLD group), 
      diesel exhaust without particles (particles, 0.01 mg/m(3); nitrogen dioxide, 1.1 
      ppm: DEG group), or clean air (pollen and control groups) for 16 h/day, 5 days/wk 
      for 24 wk, as well as to Japanese cedar pollen (JCP) (around 550,000 grains of 
      JCP/m(3)) for 2 days/wk in the same period. The control group was exposed to 
      clean air alone throughout the experiment. The mean values for Japanese cedar 
      pollen allergens (JCPAs)-specific immunoglobulin E (IgE) antibody titers in mice 
      sera measured by enzyme-linked immunosorbent assay (ELISA) in the DE, KLD, and 
      DEG groups were higher than that for the pollen alone group, but not 
      significantly, after both 12 and 24 wk of exposure time. The percentages of 
      animals expressing more than the minimum ELISA titer of JCPAs-specific IgE 
      antibodies in each group were 22% (DE and pollen groups) and 27% (KLD and DEG 
      groups) of the totals at wk 12, and no statistical differences were observed 
      among the groups. However, at wk 24 in the DE, KLD, and DEG groups the responders 
      comprised 73%, 63%, and 67%, respectively, significantly higher than the 33% for 
      the pollen alone group. No significant differences were observed among the DE, 
      KLD, and DEG groups. A slight dose-dependent increase of proliferative responses 
      of mouse cervical lymph node cells to JCPAs in both DE and KLD groups was 
      observed, but not in the DEG group. Remarkable decrease of interferon-gamma and 
      significant increase of interleukin-4 in the nasal lavage fluid were apparent 
      after DE or DEG exposure, but not in the KLD group. These results suggest that 
      these air pollutants (DE, KLD, and DEG) enhance the production of IgE antibodies 
      in mice, with similar adjuvant activities in each case. Furthermore, in the early 
      phase of exposure in which sensitization occurred with exposure to pollen, the 
      fine particles and gas components are considered to have exhibited different 
      enhancing mechanisms in mice as follows: (1) The fine particles augmented 
      production of IgE antibodies through activation of T lymphocytes, and (2) the gas 
      components exhibited almost no action on T lymphocytes, but directly induced 
      disorders of the cytokine network and augmented the production of IgE antibodies.
FAU - Maejima, K
AU  - Maejima K
AD  - Energy and Environment Research Division, Japan Automobile Research Institute, 
      2530 Karima, Tsukuba-shi, Ibaraki 305-0822, Japan. kmae@jari.or.jp
FAU - Tamura, K
AU  - Tamura K
FAU - Nakajima, T
AU  - Nakajima T
FAU - Taniguchi, Y
AU  - Taniguchi Y
FAU - Saito, S
AU  - Saito S
FAU - Takenaka, H
AU  - Takenaka H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (Air Pollutants)
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Dust)
RN  - 0 (Gases)
RN  - 0 (Soil)
RN  - 0 (Vehicle Emissions)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Air Pollutants/*adverse effects/immunology
MH  - Allergens
MH  - Animals
MH  - Antibody Formation
MH  - *Cedrus
MH  - Cytokines/*biosynthesis/drug effects
MH  - Dust/*adverse effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gases/pharmacology
MH  - Immunoglobulin E/*biosynthesis
MH  - Inhalation Exposure
MH  - Lymphocyte Activation
MH  - Mice
MH  - Particle Size
MH  - Pollen/*adverse effects/immunology
MH  - Soil
MH  - T-Lymphocytes/immunology
MH  - Vehicle Emissions/*adverse effects
EDAT- 2001/11/07 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/07 10:00
PHST- 2001/11/07 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/07 10:00 [entrez]
AID - 10.1080/089583701753210407 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2001 Nov;13(11):1047-63. doi: 10.1080/089583701753210407.