PMID- 11696988
OWN - NLM
STAT- MEDLINE
DCOM- 20020225
LR  - 20190921
IS  - 0300-8207 (Print)
IS  - 0300-8207 (Linking)
VI  - 42
IP  - 1
DP  - 2001
TI  - An elevated number of differentiated osteoblast colonies can be obtained from rat 
      bone marrow stromal cells using a gradient isolation procedure.
PG  - 49-58
AB  - Bone marrow stromal cells (BMSCs) for osteoblast differentiation studies can be 
      obtained by gradient isolation techniques or by directly plating a filtered cell 
      suspension. We compared these two procedures to evaluate whether this step is 
      critical in order to obtain a high number of differentiated colonies. Isolated 
      primary rat BMSCs were cultured in vitro with or without insulin-like growth 
      factor II (IGFII), basic fibroblast growth factor (b-FGF), epidermal growth 
      factor (EGF) or transforming growth factor beta 1 (TGF beta 1), and 
      histochemically and biochemically analysed at different time points. The gradient 
      procedure produced a significantly higher number of colonies capable of 
      osteoblastic differentiation. The growth factors had different effects. In 
      particular, b-FGF and EGF significantly increased the number of Alizarin red S 
      positive colonics, while IGFII and TGF beta I exerted inhibitory effects. Nodules 
      obtained on day 21 showed some alkaline phosphatase positive cells and were Von 
      Kossa-positive. These data demonstrate that more differentiated colonies are 
      obtainable from BMSCs isolated by the gradient procedure.
FAU - Lisignoli, G
AU  - Lisignoli G
AD  - Laboratorio di Immunologia e Genetica, I.O.R., Via di Barbiano 1/10, 40136 
      Bologna, Italy.
FAU - Remiddi, G
AU  - Remiddi G
FAU - Cattini, L
AU  - Cattini L
FAU - Cocchini, B
AU  - Cocchini B
FAU - Zini, N
AU  - Zini N
FAU - Fini, M
AU  - Fini M
FAU - Grassi, F
AU  - Grassi F
FAU - Piacentini, A
AU  - Piacentini A
FAU - Facchini, A
AU  - Facchini A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Connect Tissue Res
JT  - Connective tissue research
JID - 0365263
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67763-97-7 (Insulin-Like Growth Factor II)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Bone Marrow Cells/*cytology/drug effects
MH  - Cell Count
MH  - Cell Differentiation
MH  - Cell Separation/*methods
MH  - Cells, Cultured
MH  - Centrifugation, Density Gradient/*methods
MH  - Epidermal Growth Factor/pharmacology
MH  - Fibroblast Growth Factor 2/pharmacology
MH  - Insulin-Like Growth Factor II/pharmacology
MH  - Male
MH  - Osteoblasts/*cytology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Stromal Cells/*cytology/drug effects
MH  - Transforming Growth Factor beta/pharmacology
MH  - Transforming Growth Factor beta1
EDAT- 2001/11/08 10:00
MHDA- 2002/02/28 10:01
CRDT- 2001/11/08 10:00
PHST- 2001/11/08 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2001/11/08 10:00 [entrez]
AID - 10.3109/03008200109014248 [doi]
PST - ppublish
SO  - Connect Tissue Res. 2001;42(1):49-58. doi: 10.3109/03008200109014248.