PMID- 11698170
OWN - NLM
STAT- MEDLINE
DCOM- 20020131
LR  - 20190916
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 15
IP  - 6
DP  - 2001 Dec
TI  - Role of ATP and glycogen reserves in both paracetamol sulfation and 
      glucuronidation by cultured precision-cut rat liver slices.
PG  - 683-90
AB  - Precision-cut rat liver slices (PCLS) were used to investigate the formation of 
      paracetamol conjugates. The time course of biochemical markers such as ATP and 
      GSH content, glycogen levels and protein synthesis rates was recorded over a 
      period of time of 26 h and taken as index of slices viability. Low values of ATP 
      (3.6 nmol/mg prot), GSH (7.1 nmol/mg prot) and protein synthesis rates (94.1 pmol 
      leu/mg prot x min(-1)) were initially observed. Thereafter, they gradually 
      recovered up to 6 h but decreased values were seen after 20 h. Glycogen, however, 
      dropped rapidly during the first 6 h, being no longer detected after 20 h of 
      incubation. The reincubation of PCLS in a fresh medium for 6 h allowed a strong 
      recovery of GSH, ATP and protein synthesis rates, but no gluconeogenesis was 
      observed. Meanwhile, paracetamol sulfate formation was fairly constant (about 3 
      microg/mg protein) while glucuronide gradually disappeared. The amount of both 
      UGT1A1 and ST1A1 did not correlate with their respective enzymatic activities. We 
      suggest that loss of glycogen impair glucuronide conjugation by decreasing the 
      availability of UDPGA, and that low values of ATP are largely enough to support 
      sulfotransferase activity.
FAU - Evdokimova, E
AU  - Evdokimova E
AD  - Unite de Pharmacocinetique, Metabolisme, Nutrition et Toxicologie, Departement de 
      Sciences Pharmaceutiques, Universite Catholique de Louvain, Brussels, Belgium.
FAU - Taper, H
AU  - Taper H
FAU - Buc Calderon, P
AU  - Buc Calderon P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Analgesics, Non-Narcotic)
RN  - 0 (Glucuronides)
RN  - 0 (Ugt1a1 protein, rat)
RN  - 2616-64-0 (Uridine Diphosphate Glucuronic Acid)
RN  - 362O9ITL9D (Acetaminophen)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9005-79-2 (Glycogen)
RN  - EC 2.4.1.- (UGT1A1 enzyme)
RN  - EC 2.4.1.17 (Glucuronosyltransferase)
RN  - EC 2.8.2.1 (Arylsulfotransferase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Acetaminophen/*metabolism
MH  - Adenosine Triphosphate/analysis/*metabolism
MH  - Analgesics, Non-Narcotic/*metabolism
MH  - Animals
MH  - Arylsulfotransferase/analysis/*metabolism
MH  - Glucuronides/metabolism
MH  - Glucuronosyltransferase/analysis/*metabolism
MH  - Glutathione/metabolism
MH  - Glycogen/analysis/*metabolism
MH  - Immunoblotting
MH  - In Vitro Techniques
MH  - Liver/chemistry/drug effects/*enzymology
MH  - Male
MH  - Protein Biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Uridine Diphosphate Glucuronic Acid/metabolism
EDAT- 2001/11/08 10:00
MHDA- 2002/02/01 10:01
CRDT- 2001/11/08 10:00
PHST- 2001/11/08 10:00 [pubmed]
PHST- 2002/02/01 10:01 [medline]
PHST- 2001/11/08 10:00 [entrez]
AID - S0887-2333(01)00091-1 [pii]
AID - 10.1016/s0887-2333(01)00091-1 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2001 Dec;15(6):683-90. doi: 10.1016/s0887-2333(01)00091-1.